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Molecular Cloning of the Superoxide Dismutase Gene from Orientia tsutsugamushi, the Causative Agent of Scrub Typhus  

Koh, Young-Sang (Department of Biology, College of Natural Sciences, Cheju National University)
Yun, Ji-Hyun (Department of Biolog, College of Natural Sciences, Cheju National University)
Kim, Se-Jae (Departmemt of Microbiology, Cheju National University Medical School, Jeju 690-756, Republic of Korea)
Publication Information
Journal of Microbiology / v.40, no.2, 2002 , pp. 151-155 More about this Journal
Abstract
A Superoxide Dismutase (SOD) gene from the obligate intracellular bacterium Orientia tsutsugamushi has been cloned by using the polymerase chain reaction with degenerate oligonucleotide primers corresponding to conserved regions of known SODs. Nucleotide sequencing revealed that the predicted amino acid sequence was significantly more homologous to known iron-containing SODs (FeSOD) than to manganese-containing SODs (MnSOD). Conserved regions in bacterial FeSOD could also be seen. Isolation of the oriential SOD gene may provide an opportunity to examine its role in the intracellular survival of this bacterium.
Keywords
Orientia tsutsugamushi; superoxide dismutase; gene cloning; PCR; intracellular survival;
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