Browse > Article
http://dx.doi.org/10.3346/jkms.2015.30.7.979

Antiangiogenic Activity of Acer tegmentosum Maxim Water Extract in Vitro and in Vivo  

Kim, Eok-Cheon (Division of Biological Science and Technology and Yonsei-Fraunhofer Medical Device Lab., College of Science and Technology, Yonsei University)
Kim, So Hun (Division of Endocrinology and Metabolism, Department of Internal Medicine, Inha University School of Medicine)
Piao, Shan-Ji (Qingdao Endocrine & Diabetes Hospital)
Kim, Tack-Joong (Division of Biological Science and Technology and Yonsei-Fraunhofer Medical Device Lab., College of Science and Technology, Yonsei University)
Bae, Kiho (Division of Biological Science and Technology and Yonsei-Fraunhofer Medical Device Lab., College of Science and Technology, Yonsei University)
Kim, Han Sung (Department of Biomedical Engineering, Institute of Medical Engineering and Yonsei-Fraunhofer Medical Device Laboratory, Yonsei University)
Hong, Soon-Sun (Department of Biomedical Sciences, College of Medicine, Inha University)
Lee, Byoung Ick (Department of Obstetrics & Gynecology, Inha University College of Medicine)
Nam, Moonsuk (Division of Endocrinology and Metabolism, Department of Internal Medicine, Inha University School of Medicine)
Publication Information
Journal of Korean Medical Science / v.30, no.7, 2015 , pp. 979-987 More about this Journal
Abstract
Angiogenesis, the formation of new blood vessels, is critical for tumor growth and metastasis. Notably, tumors themselves can lead to angiogenesis by inducing vascular endothelial growth factor (VEGF), which is one of the most potent angiogenic factors. Inhibition of angiogenesis is currently perceived as one of the most promising strategies for the blockage of tumor growth. In this study, we investigated the effects of Acer tegmentosum maxim water extract (ATME) on angiogenesis and its underlying signal mechanism. We studied the antiangiogenic activity of ATME by using human umbilical vein endothelial cells (HUVECs). ATME strongly inhibited VEGF-induced endothelial cell proliferation, migration, invasion, and tube formation, as well as vessel sprouting in a rat aortic ring sprouting assay. Moreover, we found that the p44/42 mitogen activated protein (MAP) kinase signaling pathway is involved in the inhibition of angiogenesis by ATME. Moreover, when we performed the in vivo matrigel plug assay, VEGF-induced angiogenesis was potently reduced when compared to that for the control group. Taken together, these results suggest that ATME exhibits potent antiangiogenic activity in vivo and in vitro and that these effects are regulated by the extracellular regulated kinase (ERK) pathway.
Keywords
Angiogenesis; Acer tegmentosum Maxim; Vascular Endothelial Growth Factor A; p44/42 MAP Kinase;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Folkman J, Cotran R. Relation of vascular proliferation to tumor growth. Int Rev Exp Pathol 1976; 16: 207-48.
2 Folkman J, Shing Y. Angiogenesis. J Biol Chem 1992; 267: 10931-4.
3 Reinke JM, Sorg H. Wound repair and regeneration. Eur Surg Res 2012; 49: 35-43.   DOI
4 Veikkola T, Alitalo K. VEGFs, receptors and angiogenesis. Semin Cancer Biol 1999; 9: 211-20.   DOI
5 McMahon G. VEGF receptor signaling in tumor angiogenesis. Oncologist 2000; 5: 3-10   DOI
6 Senger DR, Perruzzi CA, Feder J, Dvorak HF. A highly conserved vascular permeability factor secreted by a variety of human and rodent tumor cell lines. Cancer Res 1986; 46: 5629-32.
7 Plate KH, Breier G, Weich HA, Risau W. Vascular endothelial growth factor is a potential tumour angiogenesis factor in human gliomas in vivo. Nature 1992; 359: 845-8.   DOI
8 Kim KJ, Li B, Winer J, Armanini M, Gillett N, Phillips HS, Ferrara N. Inhibition of vascular endothelial growth factor-induced angiogenesis suppresses tumour growth in vivo. Nature 1993; 362: 841-4.   DOI
9 Manley PW, Bold G, Brüggen J, Fendrich G, Furet P, Mestan J, Schnell C, Stolz B, Meyer T, Meyhack B, et al. Advances in the structural biology, design and clinical development of VEGF-R kinase inhibitors for the treatment of angiogenesis. Biochim Biophys Acta 2004; 1697: 17-27.   DOI
10 Sagar SM, Yance D, Wong RK. Natural health products that inhibit angiogenesis: a potential source for investigational new agents to treat cancer-Part 1. Curr Oncol 2006; 13: 14-26.
11 Huang S, Yang N, Liu Y, Hu L, Zhao J, Gao J, Li Y, Li C, Zhang X, Huang T. Grape seed proanthocyanidins inhibit angiogenesis via the downregulation of both vascular endothelial growth factor and angiopoietin signaling. Nutr Res 2012; 32: 530-6.   DOI
12 Lee SJ, Park K, Ha SD, Kim WJ, Moon SK. Gleditsia sinensis thorn extract inhibits human colon cancer cells: the role of ERK1/2, G2/M-phase cell cycle arrest and p53 expression. Phytother Res 2010; 24: 1870-6.   DOI
13 Yi JM, Park JS, Oh SM, Lee J, Kim J, Oh DS, Bang OS, Kim NS. Ethanol extract of Gleditsia sinensis thorn suppresses angiogenesis in vitro and in vivo. BMC Complement Altern Med 2012; 12: 243.   DOI
14 Ahn DK. Illustrated book of Korean medicinal herbs. Seoul: Kyohak Publishing Co, 1998.
15 Morikawa T, Tao J, Toguchida I, Matsuda H, Yoshikawa M. Structures of new cyclic diarylheptanoids and inhibitors of nitric oxide production from Japanese folk medicine Acer nikoense. J Nat Prod 2003; 66: 86-91.   DOI
16 Yonezawa T, Lee JW, Akazawa H, Inagaki M, Cha BY, Nagai K, Yagasaki K, Akihisa T, Woo JT. Osteogenic activity of diphenyl ether-type cyclic diarylheptanoids derived from Acer nikoense. Bioorg Med Chem Lett 2011; 21: 3248-51.   DOI
17 Barbehenn RV, Jones CP, Karonen M, Salminen JP. Tannin composition affects the oxidative activities of tree leaves. J Chem Ecol 2006; 32: 2235-51.   DOI
18 Park KM, Yang MC, Lee KH, Kim KR, Choi SU, Lee KR. Cytotoxic phenolic constituents of Acer tegmentosum maxim. Arch Pharm Res 2006; 29: 1086-90.   DOI
19 Jaffe EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 1973; 52: 2745-56.   DOI
20 Lee OH, Kim YM, Lee YM, Moon EJ, Lee DJ, Kim JH, Kim KW, Kwon YG. Sphingosine 1-phosphate induces angiogenesis: its angiogenic action and signaling mechanism in human umbilical vein endothelial cells. Biochem Biophys Res Commun 1999; 264: 743-50.   DOI
21 Nicosia RF, Ottinetti A. Modulation of microvascular growth and morphogenesis by reconstituted basement membrane gel in three-dimensional cultures of rat aorta: a comparative study of angiogenesis in matrigel, collagen, fibrin, and plasma clot. In Vitro Cell Dev Biol 1990; 26: 119-28.   DOI
22 Lal BK, Varma S, Pappas PJ, Hobson RW 2nd, Duran WN. VEGF increases permeability of the endothelial cell monolayer by activation of PKB/akt, endothelial nitric-oxide synthase, and MAP kinase pathways. Microvasc Res 2001; 62: 252-62.   DOI
23 Ali N, Yoshizumi M, Fujita Y, Izawa Y, Kanematsu Y, Ishizawa K, Tsuchiya K, Yano S, Sone S, Tamaki T. A novel Src kinase inhibitor, M475271, inhibits VEGF-induced human umbilical vein endothelial cell proliferation and migration. J Pharmacol Sci 2005; 98: 130-41.   DOI
24 Lee SJ, Namkoong S, Kim YM, Kim CK, Lee H, Ha KS, Chung HT, Kwon YG, Kim YM. Fractalkine stimulates angiogenesis by activating the Raf-1/MEK/ERK- and PI3K/Akt/eNOS-dependent signal pathways. Am J Physiol Heart Circ Physiol 2006; 291: H2836-46.   DOI
25 Birney YA, Sweeney CH, Cappadona CR, Sitzmann JV, Cummins PM, Redmond EM, Cahill PA. Pulse pressure-induced transmural fluid flux increases bovine aortic smooth muscle cell apoptosis in a mitogen activated protein kinase dependent manner. J Vasc Res 2004; 41: 364-74.   DOI
26 Beckner ME. Factors promoting tumor angiogenesis. Cancer Invest 1999; 17: 594-623.   DOI
27 Junttila MR, Li SP, Westermarck J. Phosphatase-mediated crosstalk between MAPK signaling pathways in the regulation of cell survival. FASEB J 2008; 22: 954-65.   DOI
28 Huang C, Jacobson K, Schaller MD. MAP kinases and cell migration. J Cell Sci 2004; 117: 4619-28.   DOI
29 Finlay D, Healy V, Furlong F, O'Connell FC, Keon NK, Martin F. MAP kinase pathway signalling is essential for extracellular matrix determined mammary epithelial cell survival. Cell Death Differ 2000; 7: 302-13.   DOI
30 Rousseau S, Houle F, Landry J, Huot J. p38 MAP kinase activation by vascular endothelial growth factor mediates actin reorganization and cell migration in human endothelial cells. Oncogene 1997; 15: 2169-77.   DOI
31 Chrzanowska-Wodnicka M, Kraus AE, Gale D, White GC 2nd, Vansluys J. Defective angiogenesis, endothelial migration, proliferation, and MAPK signaling in Rap1b-deficient mice. Blood 2008; 111: 2647-56.   DOI
32 Ziche M, Morbidelli L, Masini E, Amerini S, Granger HJ, Maggi CA, Geppetti P, Ledda F. Nitric oxide mediates angiogenesis in vivo and endothelial cell growth and migration in vitro promoted by substance P. J Clin Invest 1994; 94: 2036-44.   DOI
33 Babaei S, Stewart DJ. Overexpression of endothelial NO synthase induces angiogenesis in a co-culture model. Cardiovasc Res 2002; 55: 190-200.   DOI
34 Morbidelli L, Chang CH, Douglas JG, Granger HJ, Ledda F, Ziche M. Nitric oxide mediates mitogenic effect of VEGF on coronary venular endothelium. Am J Physiol 1996; 270: H411-5.   DOI
35 Kim YM, Namkoong S, Yun YG, Hong HD, Lee YC, Ha KS, Lee H, Kwon HJ, Kwon YG, Kim YM. Water extract of Korean red ginseng stimulates angiogenesis by activating the PI3K/Akt-dependent ERK1/2 and eNOS pathways in human umbilical vein endothelial cells. Biol Pharm Bull 2007; 30: 1674-9.   DOI
36 Ziche M, Morbidelli L, Choudhuri R, Zhang HT, Donnini S, Granger HJ, Bicknell R. Nitric oxide synthase lies downstream from vascular endothelial growth factor-induced but not basic fibroblast growth factor-induced angiogenesis. J Clin Invest 1997; 99: 2625-34.   DOI
37 Papapetropoulos A, García-Cardeña G, Madri JA, Sessa WC. Nitric oxide production contributes to the angiogenic properties of vascular endothelial growth factor in human endothelial cells. J Clin Invest 1997; 100: 3131-9.   DOI
38 Hood JD, Meininger CJ, Ziche M, Granger HJ. VEGF upregulates ecNOS message, protein, and NO production in human endothelial cells. Am J Physiol 1998; 274: H1054-8.
39 Lee PC, Salyapongse AN, Bragdon GA, Shears LL 2nd, Watkins SC, Edington HD, Billiar TR. Impaired wound healing and angiogenesis in eNOS-deficient mice. Am J Physiol 1999; 277: H1600-8.