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http://dx.doi.org/10.7852/ijie.2022.45.2.115

Developmental characteristics and genetic diversity of the two-spotted cricket Gryllus bimaculatus De Geer, 1773 (Orthoptera: Gryllidae) in South Korea  

Gyu-Dong, Chang (Industrial Insect and Sericulture Division, Department of Agricultural Biology, National Institute of Agricultural Sciences)
Su Hyun, Yum (Industrial Insect and Sericulture Division, Department of Agricultural Biology, National Institute of Agricultural Sciences)
Jeong-Hun, Song (Industrial Insect and Sericulture Division, Department of Agricultural Biology, National Institute of Agricultural Sciences)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.45, no.2, 2022 , pp. 115-125 More about this Journal
Abstract
In this study, we investigated the developmental characteristics and genetic diversity of seven populations of two-spotted crickets (Gryllus bimaculatus De Geer, 1773 (Orthoptera: Gryllidae)) raised in South Korea. Regarding the developmental characteristics of the species, we observed no statistically significant difference in the weight of the nymphs in the six populations we tested. After molting, although weight differences were observed between the populations in each stage of the developmental period, the average weight for each developmental stage was constant. We also analyzed mitochondrial COI gene sequences (DNA barcoding region) of the reared crickets collected from five insect farms and two national insect rearing facilities and the resultant sequences were analyzed together with the 12 sequences from foreign countries specimens obtained from public data. We detected six haplotypes from 111 specimens, indicating a low intraspecific genetic distance (~1.8%). The most dominant haplotype was overwhelmingly haplotype 1, which was found in all South Korean specimens and four specimens from China, Indonesia, and Germany. These findings indicate that the low genetic diversity of South Korean specimens can be explained by the fact that the G. bimaculatus population imported for feed from Japan in the early 2000s became a maternal group that spread throughout cricket farms in South Korea. In order to breed healthy cricket strains, it is necessary to increase genetic diversity by importing them from other countries through appropriate quarantine procedures.
Keywords
Gryllus bimaculatus; genetic diversity; COI; haplotype; developmental characteristics; Korea;
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