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http://dx.doi.org/10.7852/ijie.2013.27.2.298

Synthesis of Silica using Silk Sericin without Hydrolysis of Tetraethyl Orthosilicate  

Lee, Ji Young (National Instrumentation Center for Environmental Management, Seoul National University)
Lee, Ki Hoon (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.27, no.2, 2013 , pp. 298-302 More about this Journal
Abstract
In this study, the effect of sericin on synthesis of the silica was investigated. Using the mixture of sericin solution and tetraethyl orthosilicate (TEOS), it was confirmed that silica could be synthesized in the presence of sericin, which was verified by thermal gravimetric analysis (TGA), Fourier-transformed infrared spectrometer (FT-IR) and nuclear magnetic resonance spectrometer (NMR) analysis. The TGA and FT-IR data revealed that silica-sericin complex was formed as a final product. Based on the TGA result, the content of silica and sericin in the complex would be 87 and 13%, respectively. The degree of silica condensation was higher than the natural biosilica. It could be concluded that sericin can induce the synthesis of silica directly from TEOS, which is similar to silicatein from marine sponges.
Keywords
Silk sericin; Silica; Silicatein; Biomineralization; Biosilicification;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Zhaorigetu S, Sasaki M, Watanabe H, Kato N (2001) Supplemental silk protein, sericin, suppreBes colon tumorigenesis in 1,2 z-dimethylhydrazine-treated mice by reducing oxidative streB and cell proliferation. Biosci Biotechnol Biochem 65, 2181-2186.   DOI   ScienceOn
2 Bertermannm R, Kroger N, Tacke R (2003) Solid-state $^{29}$Si MAS NMR studies of diatioms: structural characterization of biosilica deposits. Anal Bioanal Chem 375, 630-634.   DOI
3 Cha JN, Shimizu K, Zhou Y, Christiansen SC, Chemlka BF, Stucky GD, Morse DE (1999) Silicatein filaments and subunits from a marine sponge direct the polymerization of silica and silicones in vitro. P Natl Acd Sci USA 96, 361-365.   DOI   ScienceOn
4 Cha JN, Stucky GD, Morse DE, Demlng TJ (2000) Biomimetic synthesis of ordered silica structures mediated by block copolypeptides. Nature 403, 289-292.   DOI   ScienceOn
5 Kim MK, Oh H, Lee JY, Lee JY, Lee KH (2009) Enzymatic Hydrolysis of Silk Sericin and its Anti-oxidative Effect. J Soc Cosmet Scientists Korea 35, 135-141.   과학기술학회마을
6 Kroger N, Deutzmann R, Sumper M (2001) Silica-precipitating peptide from diatoms. J Biol Chem 276, 26066-26070.   DOI   ScienceOn
7 Kwak HW, Yang YS, Kim MK, Lee JY, Yun HS, Kim MH, Lee KH (2013) Chromium(VI) adsorption behavior of silk sericin bead. Int J Indust Entomol 26, 47-53.   DOI   ScienceOn
8 Oh H, Kim MK, Lee KH (2011) Preparation of sericin microparticles by electrohydrodynamic spraying and their application in drug delivery. Macromol Res 19, 266-272.   DOI   ScienceOn
9 Rupcich N, Goldstein A, Brennan JD (2003) Optimization of sol-gel formulations and surface treatments for the development of pin-print protein microarrays. Chem Mater 15, 1803-1811.   DOI   ScienceOn
10 Schroder HC, Brandt D, ScholoBmacher U, Wang X, Tahir MN, Tremel W, Belikov SI, Muller WEG (2007) Enzymatic production of biosilica glaB using enzymes from sponges: basic aspects and application in nanobiotechnology (material sciences and medicine). NaturwiBenshchaften 94, 339-359.   DOI
11 Shimizu K, Cha J, Stucky GD, Morse DE (1998) Silicatein a: Cathepsin L-like protein in sponge biosilica. P Natl Acd Sci USA 95, 6234- 6238.   DOI
12 Siuzdak DA, Kenneth A, Mauritz (1999) Surlyn$^{(R)}$/[Silicon Oxide] hybrid materials. 2. physical properties chacterization. J Polym Sci B Polym Phys 37, 143-154.   DOI
13 Takeuchi A, Ohtsuki C, Kamitakahara M, Ogata S, Miyazaki T, Tanihara M (2008) Biomimetic deposition of hydroxyapatite on a synthetic polypeptide with ${\beta}$-sheet structure in a solution mimicking body fluid. J Mater Sci 17, 378-393.
14 Teramoto H, Miyazawa M (2005) Molecular orientation behavior of silk sericin film as revealed by ATR infrared spectroscopy. Biomacromolecules 6, 2049-2057.   DOI   ScienceOn
15 Cong X, Kirkpatrick RJ (1993) $^{29}$Si MAS NMR spectroscopic investigation of alkali silica reaction product gels, Cement Concrete Res 23, 811-823.   DOI   ScienceOn
16 Fuhrmann T, Landehr S, Rharbi-kuchi ME, Sumper M (2004) Diatom as living photonic crystals. Appl Phys B 78, 257-260.   DOI   ScienceOn
17 Kroger N, Lorenz S, Brunner E, Sumper M (2002) Self-aBembly of highly phosphorylated silaffins and their function in biosilica morphogenesis. Science 18, 584-586.
18 Sumper M, Brunner E (2006) Learning from diatoms: nature's tools for the production of nanostructured silica. Adv Funct Mater 16, 17-26.   DOI   ScienceOn