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Characteristics of Silk Sericin Extracted from Sericinjam  

Kweon, Hae-Yong (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Yeo, Joo-Hong (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Kim, Kee-Young (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Kim, Yong-Soon (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Song, Ha-Seok (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Kim, Su-Jin (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Woo, Soon-Ok (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Han, Sang-Mi (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Lee, Kwang-Gill (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.18, no.2, 2009 , pp. 121-124 More about this Journal
Abstract
We investigated sericin extracted from sericinjam, which was inbred at National Academy of Agricultural Science, Suwon, Korea. Sericinjam sericin is composed of 5 fractions: 250 kDa, 120 kDa, 90 kDa, 70 kDa and 40 kDa. Amino acid analysis showed that the major amino acids of sericinjam sericin were Ser, Gly, Asp, Glu, Thr and Ala. Infrared spectra showed that sericinjam sericin has $\beta$-sheet structure. Thermal property of sericin was investigated using DSC and then they showed characteristic degradation peak at around $215{\sim}240^{\circ}C$.
Keywords
Sericinjam sericin; Amino acid; $\beta$-sheet structure; Degradation;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Dash R, Mukherjee S, Kundu SC (2006) Isolation, purification and characterization of silk protein sericin from cocoon peduncles of tropical tasar silkworm, Antheraea mylitta. Int J Biol Macromol 38, 255-258   DOI   ScienceOn
2 Dash R, Ghosh SK, Kaplan DL, Kundu SC (2007) Purification and biochemical characterization of a 70 kDa sericin from tropical tasar silkworm, Antheraea mylitta. Comparative Biochemistry and Physiology Part B 147, 129-134   DOI   ScienceOn
3 Lammli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685   DOI   PUBMED   ScienceOn
4 Lee K, Kweon H, Lee Y, Yeo J, Woo S, Cho C, Kim K (2002) Characteristics of silk sericin powder prepared by mechanical treatment. Korean J Seric Sci 44(2), 82-86
5 Sasaki M., Yamada H, Kato N (2000) Consumption of silk protein, sericin, elevates intestinal absorption of zinc, iron, magnesium and calcium in rats. Nutrition Research 20(10), 1505-1511
6 Takasu Y, Yamada H, Tsubouchi K (2002) Isolation of three main sericin components from the cocoon of the silkworm, Bombyx mori. Biosci Biotechnol Biochem 66, 2715-2718   DOI   ScienceOn
7 Kang PD, Sohn BH, Lee SU, Kim MJ, Jung IY, Kim YS, Kim YD, Lee HS (2004) Breeding of a New Silkworm Variety, Kumhwangjam, with a Sex-Limited Cocoon Color for Spring Rearing Season. Int J Indust Entomol 9(1), 89-93
8 Wu J, Wang Z, Xu S (2007) Preparation and characterization of sericin powder extracted from silk industry wastewater. Food Chemistry 103, 1255-1262   DOI   ScienceOn
9 Gamo T, Inokuchi T, Laufer H (1977) Polypeptides of fibroin and sericin secreted from different sections of the silk gland in Bombyx mori. Insect Biochem 7, 285-295   DOI   ScienceOn
10 Tamada Y, Sano M, Niwa K, Imai T, Yoshino G (2004) Sulfation of silk sericin and anticoagulant activity of sulfated sericin. J Biomater Sci Polym Ed 15, 971-980   DOI   ScienceOn
11 Teramoto H, Katazu A, Yamauchi K, Asakura A (2007) Role of hydroxyl side chains in Bombyx mori silk sericin in stabilizing its solid structure. Macromolecules 40, 1562-1569   DOI   ScienceOn
12 Lee K, Yeo J, Lee Y, Kweon H, Kim J (2001) Bioactive and skin-compatible properties of silk sericin. Korean J Seric Sci 43(2), 109-115
13 Kweon H, Yeo J, Lee K, Park Y, Nahm J, Cho C (2000) Effects of poloxamer on the gelation of silk sericin. Macromol Rapid Commun 21, 1302-1305   DOI   ScienceOn
14 Fabiani C, Pizzichini M, Spadori M, Zeddita G (1996) Treatment of waste water from silk degumming process for protein recovery and water reuse. Desalination 105, 1-9   DOI   ScienceOn
15 Kim YD, Kweon H, Woo S (2001) Collecting method of silk sericin from degumming solution and characteristics of recovered sericin. Korean J Seric Sci 43, 37-40
16 Kweon H, Cho C (2001) Biomedical applications of silk protein. Int J Indust Entomol 3(1), 1-6
17 Aramwit P, Sangcakul A (2007) The effects of sericin cream on wound healing in rats. Biosci Biotechnol Biochem 71(10), 2473-2477   DOI   ScienceOn
18 Zhao RS, Yanaka SN (2003) Inhibitory effects of silk protein, sericin, on UVB-induced acute damage and tumor promotion by reducing oxidative stress in the skin of hairless mouse. J Photochem Photbiol B: Biol 71, 11-17   DOI   ScienceOn
19 Oh H, Lee JY, Kim A, Ki CS, Kim JW, Park YH, Lee KH (2007) Preparation of silk sericin beads using LiCl/DMSO solvent and their potential as a drug carrier for oral administration. Fibers and Polymers 8(5), 470-475   DOI   ScienceOn
20 Kato N, Sato S, Yamanaka A, Yamada H, Fuwa N, Nomura M (1998) Silk protein, sericin, inhibits lipid peroxidation and tyrosinase activity. Biosci Biotechnol Biochem 62, 145-147   DOI   ScienceOn
21 Lee K, Kweon H, Yeo J, Woo S, Lee Y, Cho C, Kim K, Park Y (2003) Effect of methyl alcohol on the morphology and conformational characteristics of silk sericin. Int J Biol Macromol 33, 75-80   DOI   ScienceOn
22 Kweon H, Park YH (1994) Structural characteristics and physical properties of wild silk fibers: Antheraea pernyi and Antheraea yamamai. Korean J Seric Sci 36(2), 138-146