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Antioxidant Activity of N-hydroxyethyl Adenosine from Isaria sinclairii  

Ahn, Mi-Young (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Heo, Jung-Eun (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Ryu, Jae-Ha (College of Pharmacy, Sookmyung Women's University)
Jeong, Hy-Kyoung (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Ji, Sang-Deok (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Park, Hae-Chul (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Sim, Ha-Sik (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.17, no.2, 2008 , pp. 197-200 More about this Journal
Abstract
The antioxidant activity of Isaria (Paecilomyces) sinclairii was determined by measuring its radical scavenging effect on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The n- BuOH extract of P. sinclairii showed strong scavenging activity to DPPH. The anti-oxidant potential of the individual fraction was in the order of ethylacetate> n- BuOH>chloroform>n-hexane. The n-BuOH soluble fraction exhibiting strong anti-oxidant activity was further purified by repeated silica gel column chromatography. N-(2-Hydroxyethyl)adenosine (HEA) was isolated as one of the active principles from the n-BuOH layer. The n-BuOH layer, particularly HEA, did not increase the level of nitric oxide (NO) production in vascular endothelial cells that might be related to vasorelaxation such as the action of viagra. In addition, the vascular endothelial growth factor (VEGF) levels showed little or no increase compared with control group with the treatment of I. sinclairii.
Keywords
Isaria sinclairii; N-(2-Hydroxyethyl)adenosine; Antioxidant activity;
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1 Ahn, M. Y., Y. S. Jung, S. D. Jee, C. S. Kim, S. H. Lee, C. H. Moon, S. I. Cho, B. M. Lee and K. S. Ryu (2007a) Antihypertensive effect of the Dongchunhacho, Isaria sinclairii, in the spontaneously hypertensive rats. Arch. Pharm. Res. 30, 493-501   과학기술학회마을   DOI
2 Fukai, T., K. Satoh, T. Nomura and H. Sakagami (2003) Antinephritis and radical scavenging activity of preylflavonoids. Fitoterapia 74, 720-724   DOI   ScienceOn
3 Kim, S. Y., J. J. Gao, W. C. Lee, K. S. Ryu, K. R. Lee and Y. C. Kim (1999) Antioxidative flavonoids from the leaves of Morus alba. Arch. Pharm. Res. 22, 81-85   DOI   ScienceOn
4 Nims, R. W., J. F. Darbyshire and J. E. Saavedra (1995) Colorimetric methods for the determination of nitric oxide concentration in neutral aqueous solutions. Methods 7, 48-54   DOI   ScienceOn
5 Son, K. H., J. C. Do and S. S. Kang (1991) Isolation of adenosine from Rhizomes of Polygonatum sibiricum. Arch. Pharm. Res., 14, 193-194   DOI
6 Blois, M. S. (1958) Antioxidant determinations by the use of stable free radicals. Nature 26, 1199-1200
7 Ahn, M. Y., S. D. Jee, J. Y. Kim, J. W. Han, Y. K. Lee, Y. W. Lee, K. S. Ryu, B. M. Lee, N. J. Jung and S. N. Kim (2004) Thirteen-week repeated oral toxicity study of Paecilomyces sinclairii in Sprague-Dawely rats. J. Toxicol. Pub. Health 20, 339-348
8 Choi, J. W., B. K. Yoo, M. K. Ryu, M. S. Choi, G. H. Park and K. H. Ko (2005) Adenosine and purine prevent the disruption of mitochondrial transmembrane potential by peroxynitrite in rat primary asrocytes, Arch. Pharm. Res. 28, 810-815   과학기술학회마을   DOI
9 Geldof, A. A., S. C. Mastbergen, R. E. Henrar and G. T. Faircloth (1999) Cytotoxicity and neurocytotoxicity of new marine anticancer agents evaluated using in vitro assays. Cancer Chemother. Pharmacol. 44, 312-318   DOI   ScienceOn
10 Lee, S. J. and H. K. Lee (2005) Sanguiin H-6 blocks endothelial cell growth through inhibition of VEGF binding to WEGF receptor. Arch Phram Res. 11, 1270-1274   과학기술학회마을   DOI
11 Wang, G. J., H. W. Tseng, C. J. Chou, T. H. Tsai, C. T. Chen and M. K. Lu (2003) The vasorelaxation of Antrodia camphorata mycelia: Involvement of endothelial $Ca^{2+}$-NO-cGMP pathway. Life Science 73, 2767-2783
12 Lioyd, H. G. E., L. Ross, K. M. Li and R. I. Ludowyke (1998) Evidence that IgE receptor stimulation increases adenosine release from rat basophilic leukaemia (RBL-2H3) cells. Pulm. Pharmacol.Ther. 11, 41-46   DOI   ScienceOn
13 Ahn, M. Y., S. D. Jee and B. M. Lee (2007b) Anti-obesity effects of Isaria sinclairii by repeated oral treatment in obese Zucker rats over a 4-month period. J. Toxicol. Environ. Health 70, 1395-1401   DOI   ScienceOn
14 Hung, L. F., Y. Z. Liang, F. Q. Guo, Z. F. Zhou and B. M. Cheng (2003) Simultaneous separation and determination of active components in Cordyceps sinensis and Coryceps militarris by LC/ESI-MS. J. Pharm. Biomed. Anal. 33, 1155-1162   DOI   ScienceOn
15 Jung, M. J., H. Y. Chung and J. S. Choi (2001) Antioxidant activity of roasted deffated Perilla seed. Nat. Prod. Sci. 7, 72-75   과학기술학회마을
16 Furuya, T., M. Hirotani and M. Matsuzuwa (1983) $N^6$-(2-hydroxyethyl)adenosine, a biologically active compound from cultured mycelia of cordyceps and Isaria species. Pytochemistry 22, 2509-2512   DOI   ScienceOn