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Molecular Characterization of A Glycine and Proline-rich Antibacterial Protein from Larvae of A Beetle, Protaetia brevitarsis  

Hwang, Jae-Sam (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Kim, Seong-Ryul (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Kang, Heui-Yun (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Yun, Eun-Young (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Ahn, Mi-Young (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Park, Kwan-Ho (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Jeon, Jae-Pil (Korea National Institute of Health)
Kim, Mi-Ae (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Kim, Nam-Jung (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Hwang, Seok-Jo (Korea National Institute of Health)
Kim, Ik-Soo (College of Agriculture & Life science, Chonnam National University)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.15, no.1, 2007 , pp. 83-85 More about this Journal
Abstract
A glycine and proline-rich antibacterial protein was cloned from larvae of a beetle, Protaetia brevitarsis. The DNAs encoded a deduced propeptide of 127 amino acid residues with predicted molecular weight of 14.0 kDa and PI of 7.89. Structural analysis of this protein indicated the presence of a recognition sequence for the cleavage site within the constitutive secretory pathway(Arg-Xaa-Lys/Arg-Arg), suggesting that mature portion(72 amino acid residues) is produced by cleavage of signal peptide and propeptide from 127 amino-acid-long precursor protein. Mature portion sequence of this protein showed 72% similarity to that of Oryctes rhinoceros Rhinocerosin and 91% to that of Holotrichia diomphalia holotricin 2. The mRNA expression was reached the highest level at 4 hrs after E. coli injection and then declined gradually.
Keywords
Protaetia brevitarsis; Glycine and prolinerich protein;
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