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Induction Patterns of Suppressor of Cytokine Signaling (SOCS) by Immune Elicitors in Anopheles sinensis  

Noh Mi-Young (Department of Agricultural Biology, College of Agriculture and Life Science, Chonnam National University)
Jo Yong-Hun (Department of Agricultural Biology, College of Agriculture and Life Science, Chonnam National University)
Lee Yong-Seok (Department of Parasitology, Inje University of Medical School)
Kim Heung-Chul (US Army, 5th Medical Detachment, 18th Medical Command)
Bang In-Seok (MyGene Bioscience Institute)
Chun Jae-Sun (Department of Biological Education, Korea National University of Education)
Lee In-Hee (Department of Bio-Technology, Hoseo University)
Seo Sook-Jae (Division of Applied Life Science, Gyeongsang National University)
Shin E-Hyun (Korean National Institute of Health, Department of Viral Diseases)
Han Man-Deuk (Department of Dental Hygiene, Kimcheon College)
Kim Ik-Soo (Department of Agricultural Biology, College of Agriculture and Life Science, Chonnam National University)
Han Yeon-Soo (Department of Agricultural Biology, College of Agriculture and Life Science, Chonnam National University)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.12, no.2, 2006 , pp. 57-61 More about this Journal
Abstract
Suppressor of cytokine signaling (SOCS) is known to be as a negative feedback regulator in Janus kinase signal transducer and activator of transcription signaling. Highly conserved SOCS box domain was cloned from a Korean malaria vector, Anopheles sinensis. Sequence analysis indicates that it has identity to Anopheles gambiae (96%), Aedes aegypti (94%), Drosophila melanogaster (78%), Mus musculus (72%) and Homo sapiens (72%), respectively. Tissue specificity RT-PCR demonstrated that the expression level of AsSOCS transcript was high at abdomen, midgut, and ovary, whereas developmental expression patterns showed that the level of AsSOCS was high at egg, early pupae, and adult female. On the other hand, RT-PCR analysis after bacterial challenge showed that SOCS mRNA was strongly induced in larvae. In addition, it was also induced by various immune elicitors such as lipoteicoic acid, CpG-DNA, and laminarin. It seems that AsSOCS, repressor of JAK-STAT pathway, is highly conserved in mosquito, and may play an important role in mosquito innate immune response.
Keywords
Mosquito; Anopheles sinensis; SOCS; immune elicitors;
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