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Cloning and Characterization of Ribosome-associated Membrane Protein 4 (RAMP4) gene in silkworm Bombyx mori  

Yao Qin (Institute of Life Sciences, JiangSu University)
Hu Zhigang (Institute of Life Sciences, JiangSu University)
Xu Jiaping (Institute of Life Sciences, JiangSu University)
Chen Keping (Institute of Life Sciences, JiangSu University)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.10, no.2, 2005 , pp. 125-129 More about this Journal
Abstract
Ribosome-associated membrane protein 4 (RAMP4) is a membrane protein that exposes its N-terminal hydrophilic portion on the cytoplasmic side and spans the membrane close to the C-terminal end. RAMP4 has previously been reported to belong to the set of proteins that remains associated with membrane-bound ribosomes, and controls the glycosylation of major histocompatbility complex class II-associated invariant chain. RAMP4 also may be relative to the stabilization of membrane proteins in response to stress, with other components of translocon, and molecular chaperons in ER. Application of 5'-RACE technique with specially designed primer, we cloned a 715 bp cDNA fragment which contains a 195 bp ORF, termed RAMP4. The deduced protein has 64 amino acid residues and contains a putative transmembrane-spanning domain at the COOH terminus.
Keywords
RAMP4; Ribosome-associated membrane protein 4; Bombyx mori; RACE;
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