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Possible Presence of an Interleukin-6-Like Molecule in the Immunized Bombyx mori L. (Lepidoptera)  

Kim, Iksoo (Department of Sericulture and Entomology, The National Institute of Agricultural Science ＆ Technology, RDA)
Lee, Young-Shin (Department of Life Science, Hoseo University)
Lee, Joon-Ha (Department of Life Science, Hoseo University)
Kim, Sang-Hyun (Department of Sericulture and Entomology, The National Institute of Agricultural Science ＆ Technology, RDA)
Kang, Pil-Don (Department of Sericulture and Entomology, The National Institute of Agricultural Science ＆ Technology, RDA)
Lee, In-Hee (Department of Life Science, Hoseo University)
Kim, Jin-Won (Department of Sericulture and Entomology, The National Institute of Agricultural Science ＆ Technology, RDA)
Lee, Heui-Sam (Department of Sericulture and Entomology, The National Institute of Agricultural Science ＆ Technology, RDA)
Kang, Seok-Woo (Department of Sericulture and Entomology, The National Institute of Agricultural Science ＆ Technology, RDA)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.7, no.2, 2003 , pp. 165-173 More about this Journal
Abstract
Cytokines represent an essential part of the innate immune response in mammals. Recently, several studies have reported the presence of cytokine-like activities and molecules in the invertebrates such as echinoderms, tunicates, mollusks and insects. In our serial study, we investigated presence of cytokines in the silkworm, Bombyx mori, infected with several immune inducers. Western blotting analysis using rabbit anti-human cytokines showed the presence of IL-6-like molecule in the hemolymph collected at 8 and 24 hrs after infection with peptidoglycan and oligodeoxynucleotide, and the molecular weight of the proteins was ∼45 kDa. We attempted to isolate the molecule by gel permeation HPLC, anion exchange chromatography, ultra centrifugation, and immuno-dot-blot assay, but until now the effort was not much successful yet. It, however, does not appear that the IL-6-like molecule in the silkworm larvae is a mere experimental artifact happened by Western blotting analysis. Instead, further experiment on this subject probably will provide us more fruitful result as detected in other invertebrates including insects.
Keywords
Cytokines; Interleukin 6; IL-6-like molecule; Oligodeoxynucleotide; Silkworm; Bambyx mari; Invertebrate immunity;
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1 Beck, G. and G. S. Habicht (1991) Purification and biochemi-cal characterization of an invertebrate interleukin 1. Mol.Immunol. 28, 577-584   DOI   ScienceOn
2 Kim, I., S. H. Kim, Y. S. Lee, H. S. Lee, J. W. Kim, K. S. Ryu, P. D. Kang and I. H. Lee (2004) Immune stimulation in the silkworm, Bombyx mori L., by CpG oligodeoxynucleotides. Archi. Insect Biochem. Physiol. In press
3 Kishimoto, T. (1989) The biology of inter1eukin-6. Blood 74,1-10
4 Kheg, A. M., T. Wu, R. Weeratna, S. M. Efler, L. Love-Homan, L. Yang, A.-K. Yi, D. Short and H. L. Davis (1998) Sequence motifs in adenoviral DNA block immune activa-don by stimulatory CpG motifs. Proc. Natl. Acad. Sci. USA 95, 12631-12636   DOI   ScienceOn
5 King, D. R, M. D. Schrenzel, M. L. McKnight, T. H. Reidar son, K. D. Hanni, J. L. Stott and D. A. Ferrick (1996) Molec-ular cloning and sequencing of interleukin 6 cDNAfragments from the harbor seal (Phoca vitulina), killer whale (Orcinus orca), and Southem sea otter (Enhydra lutris nereis). Immunoeenetics 43, 90-195
6 Ottaviani, E., A. Franchini and C. Franceschi (1993) Presence of several cytokine-like molecules in molluscan hemocytes. Biochem. Biophys. Res. Com. 195, 984-988   DOI   ScienceOn
7 Pestahno, M., E. D. Anna, M. A. Masini and M. Sturla (1997) Localization of inteiIeukin-1 (3 mRNA in the cerebral gan-glion of the protochordate, Styela plicata. Neurosci. Lett.222, 151-154   DOI   PUBMED   ScienceOn
8 van Snick, J., S. Cayphas, J. P. Szikora, J. C. Renaluld, E. van Roost, T. Boon and R. J. Simpson (1988) cDNA cloning of murine inteiIeukm-HPl: homology with human interleukin 6. Eur. J. Immunol. 18, 193-197   DOI   ScienceOn
9 Weining, K. C., C. Sick, B. Kaspers and P. Staeheli (1998) A chicken homolog of mammalian interleukin-l beta: cDNA cloning and purification of active recombinant protein. Eur .J. Biochem. 258, 994-1000   DOI   ScienceOn
10 Wittwer, D., A. Franchini, E. Ottaviani and A. Wiesner (1999) Presence of IL-l- and TNF-like molecules in GaIleria mel-Ionella (Lepidoptera) haemocytes and in an insect cell linefrom Estigmene acraea (Lepdoptera). Cytokine 11, 637-642   DOI   ScienceOn
11 Hemandez, E. C., C. F. Suarez, J. A. Mendez, S. J. Echeverry, L. A. Murillo and M. E. Patarroyo (2002) Identification, cloning, and sequencing of different cytokine genes in four species of owl monkey. Immunogenetics 54, 645-653   DOI   ScienceOn
12 Ballas, Z. K., W. L. Rasmussen and A. M. Kheg (1996) Induc-tion of NK activity in murine and human cellls by CpG motifs in oligodeoxynucleotides and bacterial DNA. J. Immunol. 157, 1840-1845
13 Klinman, D., A.-K. Yi, S. L. Beaucage, J. Conover and A. M. Krieg (1996) CpG motifs expressed by bacterial DNA rap-idly induce lymphocytes to secrete IL-6, IL-12 and IFN. Proc. Natl Acad. Sci. USA 93, 2879-2883   DOI
14 Ottaviani, E., A. Franchini, S. Cassanelli and S. Genedani (1995) Cytokines and invertebrate immune responses. Biol.Cell 85, 87-91   DOI   ScienceOn
15 Wolff and C. Dinarello (1984) Nucleotide sequence of human monocyte mterleukin 1 precusor cDNA. Proc. Natl.Acad. Sci. USA 81, 7907-7911   DOI   ScienceOn
16 Kukielka, G. L., K. A. Youker, H. K. Hawkins, J. L. Perrard, L. H. Michael, C. M. Ballantyne, C. W. Smith and M. L. Ent-man (1994) Regulation of ICAM-l and IL-6 in myocardial ischemia: effect of reperfusion. Ann. N.Y. Acad. Sci. 723, 258-270   DOI   ScienceOn
17 Akira, S., T. Hirano, T. Taga and T. Kishimoto (1990) Biology of multifunctional cytokines: IL 6 and related molecules (IL-1 and TNF). FASEB J. 4, 2860-2867
18 Beck, G., R. Gerardo, J. J. Marchalonis and G. S. Habicht (1989) Characterization ofinterleukin-l activity in tunicates Comp. Biochem. Physiol. 92B, 93-98
19 Schneider, K., R. Klaas, B. Kaspers and P. Staeheli (2001) Chicken inter1eukin-6 cDNA structure and biological prop-erties. Eur. J. Biochem. 268, 4200-4206   DOI   ScienceOn
20 Kheg, A. M., A.-K. Yi, S. Matson, T. J. Waldschmidt, G. A BishoP, R. Teasdale, G. A. Koretzky and D. M. Klinman (1995) CpG motifs in bacterial DNA trigger direct B-cell activation. Nature 374, 546-549   DOI   ScienceOn
21 Entrala, E., J.-M. Molina-Molina, M.-J. Rosales-Lombardo, M. Santrala, E., J.-M. Molina-Molina, M.-J. Rosales-Lombardo, M. Sanchez-Moreno and C. Mascaro-Lazcano (2000) Cryptospo-ridium parvum: oocysts puhfication using potassium bromide discontinuous gradient. Veter. Parasitol. 92, 223-226   DOI   ScienceOn
22 Beck, G., R. F. O'Brien, G. S. Habicht, D. L. Stillman, E. L. Cooper and D. A. Raftos (1993) Invertebrate cytokines III: invertebrate interleukin-l-like molecules stimulate phagocy-tosis by tunicate and echinoderm cells. Cell. Immunol. 146,284-299   DOI   ScienceOn
23 Kimball, E., P. Simon, J. Saklatvala, R. Metzner and D. Wood (1986) Seriological characterization of acidic (Pl 5.4) and neutral (Pl 7.4) forms of 17 KDa and 35n kDa interleukin Lymphokine Res. 5, 119-131
24 Franchini, A., J. A. Miyan and E. Ottaviani (1996) Induction of ACTH- and TNF-alpha-like molecules in the hemocytes of Calli-phoravomitoria (Insecta, Diptera). Tissue Cell 5, 587-592
25 Raftos, D. A., E. L. Cooper, G. S. Habicht and G. Beck (1991) Invertebrate cytokines; tunicate cell proliferation stimulateby an interleukin l-like molecule. Proc. Natl. Acad. Sci. USA 88,9518-9522   DOI   ScienceOn
26 Dinarello, C. A. (1990) Cytokines as endogenous pyrogens. J. Infect. Dis. 179S2, S294-S304
27 Takakura, H., Y. Mori and M. Tatsumi (1997) Molecular clon-ing of caprine IL-6 cDNA and its expression in insect cells. Int. Arch. Allerey Immunol. 113, 409-416   DOI   ScienceOn
28 Schagger, H. and G. von Jagow (1987) Tricine sodium dodecyl sulfatepolyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 368-379   DOI   ScienceOn
29 Ohashi, T., Y. Matsumoto, T. Watari, R. Goitsuka, H. Tsujim-oto and A. Hasegawa (1993) Molecular cloning of felineinter1eukin-6 cDNA. J. Vet. Med. Sci. 55, 941-944   DOI   ScienceOn
30 Beck, G. and G. S. Habicht (1986) Isolation and charactenza-tion of a primitive interleukin-1-like protein from an inverte-brate, Asterias forbesi. Proc. Natl. Acad. Sci. USA 83, 7429-7433   DOI   ScienceOn
31 Beck, G. and G. S. Habicht (1996) Characterization of an IL-6-like molecule from an echmoderm (Asteriasforbesi). Cytok-ine 8,507-512
32 Gillespie, J. P. and M. R. Kanost (1997) Biological mediators of insect immunity. Annu. Rev. Entomol. 42, 611-643   DOI   ScienceOn
33 Swiderski, C. E., G. Sobol, D. P. Lunn and D. W. Horohov (2000) Molecular cloning, sequencing, and expression of equine ubter1eukin-6. Vet. Immunol. Immunopathol. 77, 213-220   DOI   ScienceOn
34 Towbin, H., T. Staehelin and J. Gordon (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellu-lose sheets: procedure and some applications. Proc. Natl.Acad. Sci. USA 76, 4350-4354   DOI   ScienceOn