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http://dx.doi.org/10.6109/jicce.2013.11.2.139

Shaded-Mask Filtering for Extended Depth-of-Field Microscopy  

Escobar, Isabel (Department of Applied Physics, University of Castilla la Mancha)
Saavedra, Genaro (Department of Optics, University of Valencia)
Martinez-Corral, Manuel (Department of Optics, University of Valencia)
Calatayud, Arnau (Centro de Tecnologia Fisicas, Polytechnic University of Valencia)
Doblas, Ana (Department of Optics, University of Valencia)
Abstract
This paper proposes a new spatial filtering approach for increasing the depth-of-field (DOF) of imaging systems, which is very useful for obtaining sharp images for a wide range of axial positions of the object. Many different techniques have been reported to increase the depth of field. However the main advantage in our method is its simplicity, since we propose the use of purely absorbing beam-shaping elements, which allows a high focal depth with a minimum modification of the optical architecture. In the filter design, we have used the analogy between the axial behavior of a system with spherical aberration and the transverse impulse response of a 1D defocused system. This allowed us the design of a ring-shaded filter. Finally, experimental verification of the theoretical statements is also provided.
Keywords
Aberrations; Apertures; Diffraction theory;
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