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http://dx.doi.org/10.20307/nps.2021.27.3.193

Sophora flavescens Extracts Have Therapeutic Effects on Overactive Bladder Syndrome by Potentiation of Large-Conductance Calcium-Activated Potassium Channel  

Jo, Heeji (School of Life Sciences and Center for AI-applied High Efficiency Drug Discovery, Gwangju Institute of Science and Technology (GIST))
Lee, Hyun Jun (Uimyung Research Institute for Neuroscience, Department of Pharmacy, Sahmyook University)
Jang, Sung Joo (Anti-Aging Research Institute, BIO-FD&C Co., Ltd.)
Moh, Sang Hyun (Anti-Aging Research Institute, BIO-FD&C Co., Ltd.)
Cheong, Jae Hoon (School of Pharmacy, Jeonbuk National University)
Park, Chul-Seung (School of Life Sciences and Center for AI-applied High Efficiency Drug Discovery, Gwangju Institute of Science and Technology (GIST))
Publication Information
Natural Product Sciences / v.27, no.3, 2021 , pp. 193-200 More about this Journal
Abstract
Sophora flavescens Ait. (Fabaceae) is a medicinal plant widely founded in Northeast Asia, and its dried root (Kushen) has been used as a traditional Chinese herbal medicine. The therapeutic effects of Kushen in micturition disorder was not investigated comprehensively yet. In the present study, we examined and compared the efficacy of three batches of Kushen extract using different ethanol content through an in vitro cell-based assay. Among them, we chose the batch with the highest efficacy and augmented the volume of extract for industrial purpose. The bulk extract was examined in its efficacy in the in vitro cell-based assay, and the therapeutic effects through an in vivo behavioral assay of OAB rats. The main components of the extracts were analyzed by liquid chromatography. Cytotoxicity of the extracts was investigated by MTT assay. The overall efficacy of the extract was as much as, or more than, kurarinone, a potent BKCa channel activator. Thus, the extract was a potent relaxant of urinary smooth muscle by upregulating the activity of BKCa channel. The Kushen extract could be explored as an alternative medicine against overactive bladder patients indicating severe dysfunction of BKCa channel.
Keywords
Sophora flavescens; Kushen; $BK_{Ca}$ channel; channel activator; channel modulator; overactive bladder syndrome;
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