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http://dx.doi.org/10.20307/nps.2019.25.4.317

Anti-inflammatory and Immunosuppressive Effects of Panax notoginseng  

Cao, Thao Quyen (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University)
Han, Jae Hyuk (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University)
Lee, Hyun-Su (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University)
Ha, Manh Tuan (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University)
Woo, Mi Hee (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University)
Min, Byung Sun (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University)
Publication Information
Natural Product Sciences / v.25, no.4, 2019 , pp. 317-325 More about this Journal
Abstract
Here, we designed to examine the anti-inflammatory effects on RAW264.7 cells and the immunosuppressive effects by evaluating interleukin-2 (IL-2) production in Jurkat T cells using a MeOH extract of Panax notoginseng roots. The results showed that the MeOH extract inhibited the synthesis of nitric oxide (NO) in a dose-dependent manner (IC50 value of 7.08 ㎍/mL) and displayed effects on T cell activation at a concentration of 400 ㎍/mL. In efforts to identify the potent compounds, bioactivity-guided fractionation of the MeOH extract and chemical investigation of its active CH2Cl2-, EtOAc-, and butanol-soluble fractions led to the successful isolation and identification of eleven compounds, including two polyacetylenes (1, 2), a steroid saponin (3), seven dammarane-type ginsenosides (4 - 10), and an oleanane-type ginsenoside (11). Among them, compound 11 was isolated from this plant for the first time. Compound 2 exhibited potent inhibitory effects on NO synthesis and an immunosuppressive effect with IC50 values of 2.28 and 65.57 μM, respectively.
Keywords
Panax notoginseng; anti-inflammation; immunosuppressive; ginsenoside; polyacetylene; saponin;
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