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http://dx.doi.org/10.20307/nps.2019.25.4.311

Synergistic Effect of Flavonoids from Artocarpus heterophyllus Heartwoods on Anticancer Activity of Cisplatin Against H460 and MCF-7 Cell Lines  

Daud, Nik Nurul Najihah Nik Mat (Faculty of Medicine, Universiti Sultan Zainal Abidin)
Septama, Abdi Wira (Research Center for Chemistry, Indonesian Institute of Sciences)
Simbak, Nordin (Faculty of Medicine, Universiti Sultan Zainal Abidin)
Bakar, Nor Hidayah Abu (Faculty of Medicine, Universiti Sultan Zainal Abidin)
Rahmi, Eldiza Puji (Faculty of Medicine, UPN Veteran)
Publication Information
Natural Product Sciences / v.25, no.4, 2019 , pp. 311-316 More about this Journal
Abstract
Artocarpus heterophyllus has been used as traditional medicine. This plant is one of the sources of flavonoid. Flavonoid compounds possessed a wide range of biological properties including anticancer. This study was performed to investigate the cytotoxic effect of flavonoids from A. heterophyllus on H460 and MCF-7 cell lines. The interaction of flavonoids and cisplatin against tested cancer cells was also evaluated. MTT assay was used to determine the cytotoxic effect of flavonoid. Isobologram analysis was selected to evaluate the synergistic effect between flavonoid and cisplatin, their interaction was then confirmed using AO/PI staining method. Amongst of flavonoid compounds, artocarpin exhibited strong cytotoxic effect on both MCF-7 and H460 cell lines with IC50 values of 12.53 ㎍/mL (28.73 μM) and 9.77 ㎍/mL (22.40 μM), respectively. This compound enhanced anticancer activity of cisplatin against H460 and MCF-7. The combination produced a synergistic effect on H460 and MCF-7 cell lines with a combination index (CI) values of 0.2 and 0.18, respectively. The AO/PI stained demonstrated that the combination of artocarpin and cisplatin caused morphological changes that indicated apoptosis. Moreover, artocarpanone also significantly increased cytotoxic effect of cisplatin compared to its single concentration with CI below than 1. This result suggested the potency of flavonoid named artocarpin to enhance the anticancer activity of cisplatin on H460 and MCF-7 cell lines.
Keywords
Artocarpus heterophyllus; artocarpin; non-small lung cancer cell; breast cancer cell; cytotoxicity; synergistic;
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