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http://dx.doi.org/10.20307/nps.2019.25.2.136

Dendropanax morbifera Extract Protects Cardiomyocytes against Hypoxia/Reoxygenation Injury by Inhibition of Reactive Oxygen Species Generation and Calcium Perturbation  

Lim, Leejin (Depatment of Biomedical Sciences, Chosun University Graduate School)
Ju, Sujin (Depatment of Biomedical Sciences, Chosun University Graduate School)
Song, Heesang (Depatment of Biomedical Sciences, Chosun University Graduate School)
Publication Information
Natural Product Sciences / v.25, no.2, 2019 , pp. 136-142 More about this Journal
Abstract
Ischemia/reperfusion-induced myocardial injury is the main cause of acute myocardial infarction. Dendropanax morbifera $L{\acute{e}}veille$ has been used in traditional medicines for the treatment of various diseases such as headache, infectious diseases, and general debility. However, the effect of extract from D. morbifera (EDM) on myocardial ischemic injury is still unknown. In this study, the effects of EDM on neonatal rat cardiomyocytes with hypoxia/reoxygenation (H/R) injury were investigated. The viability of cardiomyocytes with H (30 min)/R (1 h) decreased; however, treatment with EDM significantly inhibited H/R injury-induced cardiomyocyte death. Further, we observed that reactive oxygen species (ROS) generation and intracellular calcium concentration ($Ca^{2+}{_i}$) were significantly reduced in EDM-treated cardiomyocytes compared with that in H/R-injured positive control. In addition, western blotting results showed that EDM attenuated abnormal changes of RyR2 and SERCA2a genes in hypoxic cardiomyocytes. These results suggest that EDM ameliorates ROS generation and $Ca^{2+}{_i}$ homeostasis to prevent dysregulation of calcium regulatory proteins in the heart, thereby exerting cardioprotective effects and reducing hypoxia-induced cardiomyocyte damage, which verifies the potential use of EDM as a new therapeutic agent for the treatment of myocardial ischemic injury.
Keywords
Dendropanax morbifera; hypoxia/reoxygenation; cardiomyocytes;
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1 Hausenloy, D. J.; Yellon, D. M. J. Clin. Invest. 2013, 123, 92-100.   DOI
2 Cadenas, S.; Aragones, J.; Landazuri, M. O. Cardiovasc. Res. 2010, 88, 219-228.   DOI
3 Sabharwal, S. S.; Schumacker, P. T. Nat. Rev. Cancer 2014, 14, 709-721.   DOI
4 Sugamura, K.; Keaney, J. F. Jr. Free Radic. Biol. Med. 2011, 51, 978-992.   DOI
5 Vina, J.; Borras, C.; Abdelaziz, K. M.; Garcia-Valles, R.; Gomez-Cabrera, M. C. Antioxid. Redox Signal. 2013, 19, 779-787.   DOI
6 Duchen, M. R.; Verkhratsky, A.; Muallem, S. Cell Calcium 2008, 44, 1-5.   DOI
7 Yu, H. Y.; Kim, K. S.; Lee, Y. C.; Moon, H. I.; Lee, J. H. Evid. Based Complement. Alternat. Med. 2012, 2012, 637512.
8 Choi, K. M.; Zhong, Y.; Hoit, B. D.; Grupp, I. L.; Hahn, H.; Dilly, K. W.; Guatimosim, S.; Lederer, W. J.; Matlib, M. A. Am. J. Physiol. Heart Circ. Physiol. 2002, 283, H1398-H1408.   DOI
9 Boudina, S.; Abel, E. D. Circulation. 2007, 115, 3213-3223.   DOI
10 Trost, S. U.; Belke, D. D.; Bluhm, W. F.; Meyer, M.; Swanson, E.; Dillmann, W. H. Diabetes 2002, 51, 1166-1171.   DOI
11 Kain, V.; Kumar, S.; Sitasawad, S. L. Cardiovasc. Diabetol. 2011, 10, 97.   DOI
12 Erickson, J. R.; Joiner, M. L.; Guan, X.; Kutschke, W.; Yang, J.; Oddis, C. V.; Bartlett, R. K.; Lowe, J. S.; O'Donnell, S. E.; Aykin-Burns, N.; Zimmerman, M. C.; Zimmerman, K.; Ham, A. J.; Weiss, R. M.; Spitz, D. R.; Shea, M. A.; Colbran, R. J.; Mohler, P. J.; Anderson, M. E. Cell 2008, 133, 462-474.   DOI
13 Li, Y. H.; Chung, H. C.; Liu, S. L.; Chao, T. H.; Chen, J. C. Int. Heart J. 2009, 50, 207-220.   DOI
14 Kim, W.; Kim, D. W.; Yoo, D. Y.; Jung, H. Y.; Nam, S. M.; Kim, J. W.; Hong, S. M.; Kim, D. W.; Choi, J. H.; Moon, S. M.; Yoon, Y. S.; Hwang, I. K. BMC. Complement. Altern. Med. 2014, 14, 428.   DOI
15 Lee, J. W.; Park, C.; Han, M. H.; Hong, S. H.; Lee, T. K.; Lee, S. H.; Kim, G. Y.; Choi, Y. H. Oncol. Rep. 2013, 30, 1231-1238.   DOI
16 Park, B. Y.; Min, B. S.; Oh, S. R.; Kim, J. H.; Kim, T. J.; Kim, D. H.; Bae, K. H.; Lee, H. K. J. Ethnopharmacol. 2004, 90, 403-408.   DOI
17 Moon, H. I. Hum. Exp. Toxicol. 2011, 30, 870-875.   DOI
18 Chung, I. M.; Kim, M. Y.; Park, W. H.; Moon, H. I. Pharmazie 2009, 64, 547-549.
19 Chung, I. M.; Kim, M. Y.; Park, S. D.; Park, W. H.; Moon, H. I. Phytother. Res. 2009, 23, 1634-1637.   DOI
20 Chung, I. M.; Song, H. K.; Kim, S. J.; Moon, H. I. Phytother. Res. 2011, 25, 784-786.   DOI
21 Akram, M.; Kim, K. S.; Kim, E. S.; Syed, A. S.; Kim, C. Y.; Lee, J. S.; Bae, O. N. Biol. Pharm. Bull. 2016, 39, 728-736.   DOI
22 Yu, H. Y.; Jin, C. Y.; Kim, K. S.; Lee, Y. C.; Park, S. H.; Kim, G. Y.; Kim, W. J.; Moon, H. I.; Choi, Y. H.; Lee, J. H. J. Agric. Food Chem. 2012, 60, 5400-5406.   DOI
23 Lee, J. W.; Kim, K. S.; An, H. K.; Kim, C. H.; Moon, H. I.; Lee, Y. C. PLoS One. 2013, 8, e83611.   DOI
24 Jin, C. Y.; Yu, H. Y.; Park, C.; Han, M. H.; Hong, S. H.; Kim, K. S.; Lee, Y. C.; Chang, Y. C.; Cheong, J.; Moon, S. K.; Kim, G. Y.; Moon, H. I.; Kim, W. J.; Lee, J. H.; Choi, Y. H. Int. J. Oncol. 2013, 43, 1943-1950.   DOI
25 Vandergriff, A. C.; Hensley, M. T.; Cheng, K. J. Vis. Exp. 2015, 98, e52726.
26 Buja, L. M.; Entman, M. L. Circulation 1998, 98, 1355-1357.   DOI
27 Griendling, K. K.; FitzGerald, G. A. Circulation 2003, 108, 2034-2040.   DOI
28 Schriewer, J. M.; Peek, C. B.; Bass, J.; Schumacker, P. T. J. Am. Heart Assoc. 2013, 2, e000159.   DOI
29 Barry, W. H.; Bridge, J. H. Circulation 1993, 87, 1806-1815.   DOI