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http://dx.doi.org/10.20307/nps.2019.25.1.49

Study on the Marker Steroids of New Zealand Deer (Cervus elaphus var. scoticus) Velvet Antler by UPLC-MS/MS and HPLC-PDA Methods - (II)  

Lee, Nam Kyung (Laboratory of Fundamental Research, R&D Headquarters, Korea Ginseng Corp.)
Jang, Kyoung Hwa (Laboratory of Fundamental Research, R&D Headquarters, Korea Ginseng Corp.)
Lee, Jong Tae (Laboratory of Fundamental Research, R&D Headquarters, Korea Ginseng Corp.)
Park, Hee Won (Laboratory of Fundamental Research, R&D Headquarters, Korea Ginseng Corp.)
Han, Sung Tai (Laboratory of Fundamental Research, R&D Headquarters, Korea Ginseng Corp.)
In, Gyo (Laboratory of Fundamental Research, R&D Headquarters, Korea Ginseng Corp.)
Publication Information
Natural Product Sciences / v.25, no.1, 2019 , pp. 49-58 More about this Journal
Abstract
Eleven steroid hormones (SHs: androstene-3,17-dione, estrone, ${\beta}$-estradiol, ${\alpha}$-estradiol, testosterone, dehydroepiandrosterone, $17{\acute{a}}$-hydroxyprogesterone, medroxyprogesterone, megestrol acetate, progesterone, and androsterone) were detected from New Zealand deer (Cervus elaphus var. scoticus) velvet antler (NZA, 鹿茸 ). A method for the quantification of eleven SHs was established by using ultraperformance liquid chromatography (UPLC)-MS/MS. The linearities ($R^2$ > 0.991), limits of quantification (LOQ values, 0.3 ng/mL to 23.1 ng/mL), intraday and interday precisions (relative standard deviation: RSD < 2.43%), and recovery rates (97.3% to 104.6%) for all eleven SHs were determined. In addition, a method for the quantification of three 7-oxycholesterols (7-O-CSs: 7-ketocholesterol, $7{\alpha}$-hydroxycholesterol, and $7{\beta}$-hydroxycholesterol) in the NZA was established by using an HPLC-photodiode array (PDA) method. The linearities ($R^2$ > 0.999), LOQ values (30 ng/mL to 350 ng/mL), intraday and interday precisions (RSD < 1.93%), and recovery rates (97.2% to 103.5%) for the three 7-O-CSs were determined. These quantitative methods are accurate, precise, and reproducible. As a result, it is suggested that the five steroid compounds of androstene-3,17-dione, androsterone, 7-ketocholesterol, $7{\alpha}$-hydroxycholesterol, and $7{\beta}$-hydroxycholesterol could be marker steroids of NZA. These methods can be applied to quantify or standardize the marker steroids present in NZA.
Keywords
New Zealand deer antler; marker steroid; UPLC-MS/MS; HPLC-PDA; steroid hormone; 7-oxycholesterol;
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  • Reference
1 Hattori, M.; Yang, X. W.; Kaneko, S.; Nomura, Y.; Namba, T. Jap. Soc. Pharmacog. 1989, 43, 173-176.
2 Sunwoo, H. H.; Nakano, T.; Hudson, R. J.; Sim, J. S. J. Agric. Food Chem. 1995, 43 2846-2849.   DOI
3 Lee, N. K.; Shin, H. J.; Kim, W. S.; Lee, J. T.; Park, C. K. Nat. Prod. Sci. 2014, 20, 160-169.
4 Chang, I. M. Treatise on Asian Herbal Medicines I; Haksulpyunsukwan press, Korea, 2003, pp. 90, 111, 184.
5 Ker, D. F. E.; Wang, D.; Sharma, R.; Zhang, B.; Passarelli, B.; Neff, N.; Li, C.; Maloney, W.; Quake, S.; Yang, Y. P. Stem Cell Res. Ther. 2018, 31, 292-306.   DOI
6 Lu, C.; Wang, M.; Mu, J.; Han, D.; Bai, Y.; Zhang, H. Food Chem. 2013, 141, 1796-1806.   DOI
7 Griffiths, W. J.; Abdel-Khalik, J.; Crick, P. J.; Yutuc, E.; Wang, Y. J. Steroid Biochem. Mol. Biol. 2016, 162, 4-26.   DOI
8 Lund, B. C.; Bever-Stille, K. A.; Perry, P. J. Pharmacotherapy 1999, 19, 951-956.   DOI
9 Frye, C. A. Minerva Ginecol. 2009, 61, 541-562.
10 Funder, J. W.; Krozowski, Z.; Myles, K.; Sato, A.; Sheppard, K. E.; Young, M. Recent Prog. Horm. Res. 1997, 52, 247-260.
11 Gleixner, A.; Meyer, H. H. D. Fresenius J. Anal. Chem. 1997, 357, 1198-1201.   DOI
12 Gupta, B. B. P.; Lalchhandama, K. Current Sci. 2002, 83, 1103-1111.
13 Jeanneret, F.; Tonoli, D.; Rossier, M. F.; Saugy, M.; Boccard, J.; Rudaz, S. J. Chromatogr. A 2016, 1430, 97-112.   DOI
14 McDonald, J. G.; Smith, D. D.; Stiles, A. R.; Russell, D. W. J. Lipid Res. 2012, 53, 1399-1409.   DOI
15 Yang, Y.; Shao, B.; Zhang, J.; Wu, Y.; Duan, H. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2009, 877, 489-496.   DOI
16 Rodriguez, I. R.; Alam, S.; Lee, J. W. Invest. Ophthalmol. Vis. Sci. 2004, 45, 2830-2837.   DOI
17 International Conference on Harmonization ICH (Q2) R1. Validation of Analytical Procedures Test and Methodology; ICH: Switzerland, 2005, pp 6-13.
18 Kaminski, R. M.; Marini, H.; Kim, W. J.; Rogawski, M. A. Epilepsia. 2005, 46, 819-827.   DOI