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http://dx.doi.org/10.20307/nps.2017.23.4.247

Rapid Identification of Methylglyoxal Trapping Constituents from Onion Peels by Pre-column Incubation Method  

Kim, Ji Hoon (College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University)
Kim, Myeong Il (College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University)
Syed, Ahmed Shah (College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University)
Jung, Kiwon (College of Pharmacy, CHA University)
Kim, Chul Young (College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University)
Publication Information
Natural Product Sciences / v.23, no.4, 2017 , pp. 247-252 More about this Journal
Abstract
The methylglyoxal (MGO) trapping constituents from onion (Allium cepa L.) peels were investigated using pre-column incubation of MGO and crude extract followed by HPLC analysis. The peak areas of MGO trapping compounds decreased, and their chemical structures were identified by HPLC-ESI/MS. Among major constituents in outer scale of onion, 2-(3,4-dihydroxybenzoyl)-2,4,6-trihydroxy-3(2H)-benzofuranone (2) was more effective MGO scavenger than quercetin (6) and its 4'-glucoside, spiraeoside (3). After 1 h incubation, compound 2 trapped over 90% MGO at a concentration of 0.5 mM under physiological conditions, but compounds 3 and 6 scavenged 45%, 16% MGO, respectively. HPLC-ESI/MS showed that compound 2 trapped two molecules of MGO to form a di-MGO adduct and compounds 3 and 6 captured one molecule of MGO to form mono-MGO adducts, and the positions 6 and 8 of the A ring of flavonoids were major active sites for trapping MGO.
Keywords
Allium cepa L.; Methylglyoxal trapping; Pre-column incubation; Flavonoids;
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