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http://dx.doi.org/10.20307/nps.2017.23.1.21

Diarylbutane-type Lignans from Myristica fragrans (Nutmeg) show the Cytotoxicity against Breast Cancer Cells through Activation of AMP-activated Protein Kinase  

Le, Thi Van Thu (College of Pharmacy, Chosun University)
Nguyen, Phi Hung (College of Pharmacy, Chosun University)
Choi, Hong Seok (College of Pharmacy, Chosun University)
Yang, Jun-Li (Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University)
Kang, Keon Wook (Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University)
Ahn, Sang-Gun (Department of Pathology, College of Dentistry, Chosun University)
Oh, Won Keun (Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University)
Publication Information
Natural Product Sciences / v.23, no.1, 2017 , pp. 21-28 More about this Journal
Abstract
In our program to search for new AMP-activated protein kinase (AMPK) activators from plants that exert potential anticancer property, we found that an EtOAc extract of Myristica fragrans (nutmeg) activated AMPK enzyme in human breast cancer MCF-7 cells. Two major diarylbutane-type lignans, macelignan and meso-dihydroguaiaretic acid (MDGA), were isolated as active principles from this extract. Treatment of breast cancer cells with two compounds induced cellular apoptosis, evidenced by cleavage of poly-(ADP-ribose) polymerase (PARP) and Ser 15 phosphorylation of p53. Moreover, macelignan and MDGA significantly inhibited the colony formation of MCF-7 breast cancer cells on soft agar. Intraperitoneal injection of macelignan and MDGA (20 mg/kg) suppressed the tumor growth of 4T1 mammary cancer cells. These results indicate that the chemopreventive effects of two major diarylbutane-type lignans from Myristica fragrans (nutmeg) may be associated with induction of apoptosis presumably through AMPK activation.
Keywords
Myristica fragrans; Diarylbutane lignan; MCF-7; AMP-activated protein kinase (AMPK);
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