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http://dx.doi.org/10.20307/nps.2016.22.2.134

Phytochemical Identification from Boehmeria nivea Leaves and Analysis of (-)-Loliolide by HPLC  

Cho, Sunghun (Department of Integrative Plant Science, Chung-Ang University)
Lee, Dong Gu (Department of Integrative Plant Science, Chung-Ang University)
Jung, Yong-Su (Yeong-Gwang Agricultural Technology Center)
Kim, Ho Bang (Life Sciences Research Institute, Biomedic Co., Ltd.)
Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University)
Lee, Sanghyun (Department of Integrative Plant Science, Chung-Ang University)
Publication Information
Natural Product Sciences / v.22, no.2, 2016 , pp. 134-139 More about this Journal
Abstract
Phytochemicals were isolated from leaves of the fiber crop, ramie (Boehmeria nivea, Bn), using open column chromatography and medium pressure liquid chromatography. Their structures were identified as ${\beta}$-sitosterol, (-)-loliolide, rutin, and pyrimidinedione by MS, $^1H$-, and $^{13}C$-NMR spectroscopic analysis. Among them, (-)-loliolide was isolated for the first time from B. nivea. A content analysis of (-)-loliolide in B. nivea collected from different regions and harvest times was conducted by HPLC. The highest content of (-)-loliolide was found in Bn-23 harvested in September. These results will be helpful to use the plant which harvest in September as a high content phytochemical additive in food, health supplements, and medicinal products.
Keywords
Boehmeria nivea; Urticaceae; Phytochemical; Identification; Loliolide; NMR;
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