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Inhibitory Effect of the Ethyl Acetate Fraction from Tulip Tree Leaf (Liriodendron tulipifera L.) on Adipogenesis in 3T3-L1 Cells  

Nam, Hajin (Institute of Natural Medicine, Hallym University)
Jung, Harry (Department of Medical Genetics, College of Medicine, Hallym University)
Kim, Jin Kyu (Gyeonggi Institute of Science and Technology Promotion)
Suh, Jun Gyo (Institute of Natural Medicine, Hallym University)
Publication Information
Natural Product Sciences / v.19, no.3, 2013 , pp. 263-268 More about this Journal
Abstract
The inhibitory effects of adipogenesis on ethyl acetate (EtOAc) fraction from leaves of the Tulip tree (TT) were evaluated. Exposure to TT EtOAc fraction (25~200 ${\mu}g/mL$) for a 72 hr incubation period did not significantly change cell viability. TT EtOAc fraction, with concentrations of 100 and 200 ${\mu}g/mL$, inhibited lipid accumulation in 3T3-L1 adipocytes in a dose dependent manner in adipogenesis. The expression of $PPAR{\gamma}$ and $C/EBP{\alpha}$, essential adipogenic markers, was significantly decreased when TT EtOAc fraction was added to cells for 8 days as compared with the untreated control group. These results suggest that TT EtOAc fraction might be a potential therapeutic agent as an effective, natural alternative material for obesity treatment.
Keywords
Anti-obesity; Tulip tree; 3T3-L1 cells; Adipogenesis; $PPAR{\gamma}$; $C/EBP{\alpha}$;
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Times Cited By KSCI : 6  (Citation Analysis)
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