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Quantitative Determination of Flavonoids from Stems of Spatholobus suberectus  

Park, Jinseon (School of Biotechnology, Yeungnam University)
Park, So Yeon (School of Biotechnology, Yeungnam University)
Shim, Sang Hee (School of Biotechnology, Yeungnam University)
Publication Information
Natural Product Sciences / v.19, no.2, 2013 , pp. 145-149 More about this Journal
Abstract
A simple reversed phase HPLC method was established for quantitative determination of liquiritigenin (1), genistein (2), isoliquiritigenin (3), and 7-hydroxyflavanone (4) from stems of Spatholobus suberectus Dunn (Leguminosae) using a binary gradient of $H_2O$ and MeOH as a mobile phase with UV detection at 280 nm. All calibration curves showed good linear regression ($r^2$ > 0.998) within test ranges. The detection limits of the four compounds were $0.43{\sim}1.63{\mu}g/mL$. The contents of four flavonoids (1 - 4) from the stem of S. suberectus were 6.54 mg/g, 1.66 mg/g, 6.65 mg/g, and 1.93 mg/g, respectively.
Keywords
Spatholobus suberectus; Quantitative determination; Flavonooids;
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