Browse > Article

Antioxidant Activity and Its Mechanism of Paeonia lactiflora Pall Extract  

Heo, Jee-In (Department of Biochemistry, Hallym University Medical School)
Kim, Jeong-Hyeon (Department of Biochemistry, Hallym University Medical School)
Lee, Jeong-Min (Department of Biochemistry, Hallym University Medical School)
Kim, Sung-Chan (Department of Biochemistry, Hallym University Medical School)
Park, Jae-Bong (Department of Biochemistry, Hallym University Medical School)
Kim, Jaebong (Department of Biochemistry, Hallym University Medical School)
Lee, Jae-Yong (Department of Biochemistry, Hallym University Medical School)
Publication Information
Natural Product Sciences / v.19, no.1, 2013 , pp. 49-53 More about this Journal
Abstract
Paeonia lactiflora Pall (PL) has been used as a traditional herbal medicine in China, Korea, and Japan for more 1,200 years. PL has reported to have antioxidant activity and protective effect of cells from oxidative stress, although the mechanism has not been verified. FOXO3a is a transcription factor that binds to its target gene's consensus FOXO binding site. FOXO3a protein modulates the various biological functions including cell cycle control, apoptosis, DNA repair, and ROS detoxification. Therefore, FOXO3a activity is associated with cancer, aging, diabetes, infertility, neurodegeneration, and immune system dysfunction. Here we found that FOXO3a was activated by PL extract. Transcriptional target genes such as MnSOD, p27, and GADD45 were activated by PL extract. Protein levels of MnSOD and catalase were increased, consequently, ROS level was reduced in HEF cells by PL extract. These findings suggest that PL extract has an antioxidant activity through FOXO activation and thereby activation of FOXO target genes, MnSOD and catalase.
Keywords
FOXO3a; Antioxidant; Paeonia lactiflora Pall;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Lee, S.M., Yoon, M.Y., and Park, H.R., Protective effects of Paeonia lactiflora pall on hydrogen peroxide-induced apoptosis in PC12 cells. Biosci Biotechnol Biochem. 72, 1272-1277 (2008b).   DOI   ScienceOn
2 Maiese, K., Chong, Z.Z., and Shang, Y.C., OutFOXOing disease and disability: the therapeutic potential of targeting FoxO proteins. Trends Mol Med. 14, 219-227 (2008).   DOI   ScienceOn
3 Ray, P.D., Huang, B.W., and Tsuji, Y., Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cell Signal 24, 981-990 (2012).   DOI   ScienceOn
4 Tsuboi, H., Hossain, K., Akhand, A.A., Takeda, K., Du, J., Rifa'i, M., Dai, Y., Hayakawa, A., Suzuki, H., and Nakashima, I., Paeoniflorin induces apoptosis of lymphocytes through a redox-linked mechanism. J Cell Biochem. 93, 162-172 (2004).   DOI   ScienceOn
5 Wu, H., Wei, W., Song, L., Zhang, L., Chen, Y., and Hu, X., Paeoniflorin induced immune tolerance of mesenteric lymph node lymphocytes via enhancing beta 2-adrenergic receptor desensitization in rats with adjuvant arthritis. Int Immunopharmacol. 7, 662-673 (2007).   DOI   ScienceOn
6 Yang, J.Y., Zong, C.S., Xia, W., Yamaguchi, H., Ding, Q., Xie, X., Lang, J.Y., Lai, C.C., Chang, C.J., Huang, W.C., Huang, H., Kuo, H.P., Lee, D.F., Li, L.Y., Lien, H.C., Cheng, X., Chang, K.J., Hsiao, C.D., Tsai, F.J., Tsai, C.H., Sahin, A.A., Muller, W.J., Mills, G.B., Yu, D., Hortobagyi, G.N., and Hung, M.C., ERK promotes tumorigenesis by inhibiting FOXO3a via MDM2-mediated degradation. Nat Cell Biol. 10, 138-148 (2008).   DOI   ScienceOn
7 Brunet, A., Sweeney, L.B., Sturgill, J.F., Chua, K.F., Greer, P.L., Lin, Y., Tran, H., Ross, S.E., Mostoslavsky, R., Cohen, H.Y., Hu, L.S., Cheng, H.L., Jedrychowski, M.P., Gygi, S.P., Sinclair, D.A., Alt, F.W., and Greenberg, M.E., Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase. Science 303, 2011-2015 (2004).   DOI   ScienceOn
8 Cao, W., Zhang, W., Liu, J., Wang, Y., Peng, X., Lu, D., Qi, R., Wang, Y., and Wang, H., Paeoniflorin improves survival in LPS-challenged mice through the suppression of TNF-alpha and IL-1beta release and augmentation of IL-10 production. Int Immunopharmacol. 11, 172- 178 (2011).   DOI   ScienceOn
9 Furuyama, T., Nakazawa, T., Nakano, I., and Mori, N., Identification of the differential distribution patterns of mRNAs and consensus binding sequences for mouse DAF-16 homologues. Biochem J. 349, 629-634 (2000).   DOI   ScienceOn
10 Greer, E.L., Oskoui, P.R., Banko, M.R., Maniar, J.M., Gygi, M.P., Gygi, S.P., and Brunet, A., The energy sensor AMP-activated protein kinase directly regulates the mammalian FOXO3 transcription factor. J Biol Chem. 282, 30107-30119 (2007).   DOI   ScienceOn
11 He, D.Y. and Dai, S.M., Anti-inflammatory and immunomodulatory effects of paeonia lactiflora pall., a traditional chinese herbal medicine. Front Pharmacol. 2, 10 (2011).
12 Hu, M.C., Lee, D.F., Xia, W., Golfman, L.S., Ou-Yang, F., Yang, J.Y., Zou, Y., Bao, S., Hanada, N., Saso, H., Kobayashi, R., and Hung, M.C., IkappaB kinase promotes tumorigenesis through inhibition of forkhead FOXO3a. Cell 117, 225-237 (2004).   DOI   ScienceOn
13 Huang, H. and Tindall, D.J., Dynamic FoxO transcription factors. J Cell Sci. 120, 2479-2487 (2007).   DOI   ScienceOn
14 Lee, S.C., Kwon, Y.S., Son, K.H., Kim, H.P., and Heo, M.Y., Antioxidative constituents from Paeonia lactiflora. Arch Pharm Res. 28, 775-783 (2005).   DOI
15 Kim, H.S., Yeo, E.J., Park, S.H., Park, J.I., Park, S.C., Shin, J.Y., Kim, M.J., Oh, S.J., Won, M.H., Kang, T.C., Park, J.B., Kim, J., Kim, J.I., Lee, H.Y., and Lee, J.Y., p21WAF/CIP1/SDI1 is upregulated due to increased mRNA stability during hydroxyurea-induced senescence of human fibroblasts. Mech Ageing Dev. 126, 1255-1261 (2005).   DOI   ScienceOn
16 Kim, I.D. and Ha, B.J., Paeoniflorin protects RAW 264.7 macrophages from LPS-induced cytotoxicity and genotoxicity. Toxicol In Vitro 23, 1014-1019 (2009).   DOI   ScienceOn
17 Lee, B., Shin, Y.W., Bae, E.A., Han, S.J., Kim, J.S., Kang, S.S., and Kim, D.H., Antiallergic effect of the root of Paeonia lactiflora and its constituents paeoniflorin and paeonol. Arch Pharm Res. 31, 445-450 (2008a).   DOI
18 Alaimo, A., Gorojod, R.M., and Kotler, M.L., The extrinsic and intrinsic apoptotic pathways are involved in manganese toxicity in rat astrocytoma C6 cells. Neurochem Int. 59, 297-308 (2011).   DOI   ScienceOn
19 Brunet, A., Bonni, A., Zigmond, M.J., Lin, M.Z., Juo, P., Hu, L.S., Anderson, M.J., Arden, K.C., Blenis, J., and Greenberg, M.E., Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor. Cell 96, 857-868 (1999).   DOI   ScienceOn