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Quantitative Analysis for the Quality Evaluation of Scutellariae Radix by HPLC/UVD  

Jeong, Su-Yang (College of Pharmacy, Catholic University of Daegu)
Moon, Dong-Cheul (College of Pharmacy, Chungbuk National University)
Son, Kun-Ho (College of Life Science, Andong National University)
Son, Jong-Keun (College of Pharmacy, Yeungnam University)
Min, Byung-Sun (College of Pharmacy, Catholic University of Daegu)
Woo, Mi-Hee (College of Pharmacy, Catholic University of Daegu)
Publication Information
Natural Product Sciences / v.17, no.4, 2011 , pp. 321-327 More about this Journal
Abstract
In this study, quantitative analysis was developed using HPLC/UVD for the quality evaluation of Scutellariae Radix. For quantitative analysis, six major bioactive compounds were assessed. The separation conditions employed for HPLC/UVD were optimized using Phenomenex $C_{18}$ column ($250{\times}4.6$ mm, 5 ${\mu}m$) with a gradient of solvent A (1% acetic acid of $H_2O$) and solvent B (acetonitrile : methanol : acetic acid = 70 : 30 : 1) as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 275 nm. These methods were fully validated with respect to linearity, accuracy, precision and recovery. The HPLC/UVD method was applied successfully to the quantification of six major compounds in the extract of Scutellariae Radix. The results indicate that the established HPLC/UVD method is suitable for the quantitative analysis and quality control of multicomponents in Scutellariae Radix.
Keywords
Scutellariae Radix; Scutellaria baicalensis. HPLC-UV; Quantitative analysis;
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