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Topoisomerase I and II Inhibitory Activities and Cytotoxic Constituents from the Barks of Tilia amurnesis  

Piao, Dong Gen (College of Pharmacy, Yeungnam University)
Lee, You-Jeong (College of Pharmacy, Yeungnam University)
Seo, Chang-Seob (College of Pharmacy, Yeungnam University)
Lee, Chong-Soon (Department of Biochemistry, College of Science, Yeungnam University)
Kim, Jae-Ryong (Department of Biochemistry and Molecular Biology College of Medicine, Yeungnam University)
Chang, Hyun-Wook (College of Pharmacy, Yeungnam University)
Son, Jong-Keun (College of Pharmacy, Yeungnam University)
Publication Information
Natural Product Sciences / v.17, no.3, 2011 , pp. 245-249 More about this Journal
Abstract
Eight compounds, squalene (1), friedelin (2), ${\beta}$-sitosterol (3), ${\beta}$-sitosterol-3-O-glucoside (4), ${\alpha}$-tocopherol (5), betulinic acid (6), trilinolein (7) and 1-O-(9Z,12Z-Octadecadienoyl)-3-nonadecanoyl glycerol (8), were isolated from the barks of Tilia amurensis. Their chemical structures were identified by comparing their physicochemical and spectral data with those published in the literature. These isolated compounds were examined for their inhibitory activities against topoisomerase I and II. Compound 7 showed significant inhibition of DNA topoisomerase I and II activities, with percent decreases in activity of 87 and 95%, respectively at a concentration of $100\;{\mu}M$. Compound 6 exhibited cytotoxicity against the human colon adenocarcinoma cell line (HT-29), the human breast adenocarcinoma cell line (MCF-7) and the human liver hepatoblastoma cell line (HepG-2), with $IC_{50}$ values of 20, 59 and $16\;{\mu}M$, respectively.
Keywords
Cytotoxicity; DNA topoisomerases I; DNA topoisomerases II; Tilia amurensis;
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