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A Convenient HPLC/ELSD Method for the Quantitative Analysis of Betaine in Lycium chinense  

Lee, Sang-Myung (Korea Ginseng Corp. Central Research Institute)
Park, Chae-Kyu (Korea Ginseng Corp. Central Research Institute)
Cho, Byung-Goo (Korea Ginseng Corp. Central Research Institute)
Cho, Kyoung-Shim (Korea Ginseng Corp. Central Research Institute)
Min, Byung-Sun (College of Pharmacy, Catholic University of Daegu)
Bae, Ki-Hwan (College of Pharmacy, Chungnam National University)
Publication Information
Natural Product Sciences / v.17, no.2, 2011 , pp. 104-107 More about this Journal
Abstract
In order to facilitate the quality control of betaine from the fruits of Lycium chinense, we have developed a rapid and simple method for quantitative determination. Determination was achieved on a Discovery C18 column with an isocratic solvent system of 0.32% perfluoropentanoic acid aqueous-acetonitrile at a flow-rate of 0.5 mL/min and detected an ELSD. The method was reproducible with intra- and inter-day variations of less than 6% (R.S.D). The recoveries were in the range of 90.01~100.05%. The method turned out to be fast and simple, furthermore, to have a good selectivity and sensitivity for the quantity determination of betaine in the fruits of L.chinense.
Keywords
Lycium chinense; Solanaceae; Betaine; HPLC-ELSD detector;
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1 Yang, L.L., Yen, K.Y., Kiso, Y., and Kikino, H., Antihepatotoxic actions of formosan plant drugs. J. Ethnopharmacol. 19, 103-110 (1987).   DOI   ScienceOn
2 Noguchi, M., Mochida, K., Shingu, T., Kozuka, M., and Fujitani, K., Uber die Bestandteile der chinesischen Droge "Ti-ku-'pi."I. Isolierung und Konstitution von Lyciumamid, einem neuen Dipeptid. Chem. Pharm. Bull. 32, 3584-3587 (1984).   DOI   ScienceOn
3 Selhub, J., Homocysteine metabolism. Ann. Rev. Nutrit. 19, 217-246 (1999).   DOI   ScienceOn
4 Shin, Y.G., Cho, K.H., Kim, J.M., Park, M.K., and Park, J.H., Determination of betaine in Lycium chinense fruits by liquid chromatography-electrospray ionization mass spectrometry. J. Chromatograph. A 857, 331-335 (1999).   DOI
5 Sannai, A., Fujimori, T., and Kato, K., Neutral Volatile Components of "Kukoshi" (Lycium chinense M.). Agricul. Biol. Chem. 47, 2397-2399, (1983).   DOI
6 Wendel, U. and Bremer, H.J., Betaine in the treatment of homocystinuria due to 5,10-methylenetetrahydrofolate reductase deficiency. Eur. J. Pediatrics 142, 147-150 (1984).   DOI   ScienceOn
7 Murakami, T., Nagamura, Y., and Hirano, K., The Recovering Effect of Betaine on Carbon Tetrachloride-Induced Liver Injury. J. Nutrit. Sci. Vitaminol. 44, 249-255 (1998).   DOI   ScienceOn
8 Junnila, M., Barak, A.J., Beakenhauer, H.C., and Rahko, T., Betaine reduces hepatic lipidosis induced by carbon tetrachloride in Sprague- Dawley rats. Veter. Human Toxicol. 40, 263-266 (1998).
9 Mar, M.H., Ridky, T.W., Garner, S.C., and Ziesel, S.H., A method for the determination of betaine in tissues using high performance liquid chromatography. J. Nutrit. Biochem. 6, 392-398 (1995).   DOI   ScienceOn
10 Gahl, W.A., Bernardini, I., and Chen, S., The effect of oral betaine on vertebral body bone density in pyridoxine-nonresponsive homocystinuria. J. Inherit. Metabol. Dis. 11, 291-298 (1988).   DOI   ScienceOn
11 Kikuchi, N., Matsuno, K., and Miki, K., Separation and determination of betaine in an oriental medicine by liquid chromatography. Analytica Chimica Acta 283, 338-343 (1993).   DOI   ScienceOn
12 Funayama, S., Yoshida, K., Konno, C., and Hikino, H., Structure of kukoamine A, a hypotensive principle of Lycium chinense root barks1. Tetrahedron Lett. 21, 1355-1356 (1980).   DOI   ScienceOn
13 Barak, A.J., Harriet, C., Beckenhauer, H.C., Matti, J., and Dean, J.T., Dietary Betaine Promotes Generation of Hepatic SAdenosylmethionine and Protects the Liver from Ethanol-Induced Fatty Infiltration. Alcohol. Clin. Experiment. Res. 17, 552-555 (1993).   DOI   ScienceOn