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Zanthoxylum rhetsa Stem Bark Extract Inhibits LPS-induced COX-2 and iNOS expression in RAW 264.7 Cells via the NF-${\kappa}B$ Inactivation  

Thu, Nguyen Bich (National Institute of Medicinal Materials)
Trung, Trinh Nam (College of Pharmacy, Chungnam National University)
Ha, Do Thi (College of Pharmacy, Chungnam National University)
Khoi, Nguyen Minh ( National Institute of Medicinal Materials)
Than, Nguyen Viet (Hanoi University of Pharmacy)
Soulinho, Thipthaviphone (Hanoi University of Pharmacy)
Nam, Nguyen Hai (Hanoi University of Pharmacy)
Phuong, Tran Thi (College of Pharmacy, Thai Nguyen University)
Bae, Ki-Hwan (College of Pharmacy, Chungnam National University)
Publication Information
Natural Product Sciences / v.16, no.4, 2010 , pp. 265-270 More about this Journal
Abstract
The methanol extract of Zanthoxylum rhetsa (MZRR) were evaluated for its ability to suppress the formation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. MZRR presented an inhibition of LPS-induced production of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) in RAW 264.7 macrophages. Western blotting and RT-PCR analyses demonstrated that MZRR significantly inhibited the protein and mRNA expressions of iNOS and COX-2 in LPS-activated macrophages in a dose-dependent manner. LPS-induced COX-2, iNOS, and nuclear factor kappa beta (NF-${\kappa}B$) activity were also decreased in the presence of MZRR. The production of tumor necrosis factor-$\alpha$ (TNF-$\alpha$), the mRNA expression levels of pro-inflammatory cytokines, including TNF-$\alpha$ and IL-$1{\beta}$, were reduced after MZRR administration in a dose dependent-manner. These results suggest that the MZRR extract involved in the inhibition of iNOS and COX-2 via the NF-${\kappa}B$ pathway, revealing a partial molecular basis for anti-inflammatory properties of the MZRR extract.
Keywords
Zanthoxylum rhetsa; COX-2; iNOS; NF-${\kappa}B$; RAW 264.7;
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