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Characterization of Triterpenoids, Flavonoids and Phenolic Acids in Eclipta prostrata by High-performance Liquid Chromatography/diode-array Detector/electrospray Ionization with Multi-stage Tandem Mass Spectroscopy  

Lee, Ki-Yong (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
Ha, Na-Ry (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
Kim, Tae-Bum (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
Kim, Young-Choong (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
Sung, Sang-Hyun (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
Publication Information
Natural Product Sciences / v.16, no.3, 2010 , pp. 164-168 More about this Journal
Abstract
High-performance liquid chromatographic method with diode-array detector and electrospray ionization with multi-stage tandem mass spectroscopy (HPLC/DAD/ESI-$MS^n$) was used to identify the major constituents in a methanolic extract of Eclipta prostrata. The chromatographic separation was performed on a C18 column. Acetonitrile-water was used as a mobile phase. HPLC/DAD/ESI-$MS^n$ allowed the characterization of constituents of E. prostrata, mainly triterpenoids (eclalbasaponin I, II, III, IV, VI), flavonoids (luteolin 7-O-glucoside, demethylwedelolactone, wedelolactone, luteolin, demetylwedelolactone sulfate, luteolin sulfate, apigenin sulfate) and phenolic acids (5-O-caffeoylquinic acid, 3, 4-O-dicaffeoylquinic acid, 3, 5-O-dicaffeoylquinic acid, 4, 5-Odicaffeoylquinic acid).
Keywords
Eclipta prostrata; HPLC/DAD/ESI-$MS^n$; triterpenoids; flavonoids; phenolic acids;
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