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Rhus verniciflua Stokes Attenuates Glutamate-induced Neurotoxicity in Primary Cultures of Rat Cortical Cells  

Jeong, Eun-Ju (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
Sung, Sang-Hyun (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
Kim, Jin-Woong (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
Kim, Seung-Hyun (Institute for Life Science, Elcom Science Co. Ltd.)
Kim, Young-Choong (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
Publication Information
Natural Product Sciences / v.14, no.3, 2008 , pp. 156-160 More about this Journal
Abstract
The methanolic extract of Rhus verniciflua Stokes (RVS-T) and its fractions (RVS-H, RVS-C, RVS-E and RVS-B) showed significant neuroprotective activity against glutamate-induced toxicity in primary cultures of rat cortical cells. RVS-B, which showed the most potent neuroprotective activity, was further fractionated to yield RVS-B5. Treatment of cortical cells with the RVS-T, RVS-B and RVS-B5 reduced the cellular ROS level and restored the reduced activities of glutathione reductase and SOD induced by glutamate. Although, the activity of glutathione peroxidase was not virtually changed by glutamate, RVS-B5 increased the glutathione peroxidase activity. In addition, these three tested fractions significantly restored the content of GSH which was decreased by glutamate insult in our cultures. Taken together, it could be postulated that RVS extract, in particular its fraction RVS-B5, protected neuronal cells against glutamate-induced neurotoxicity through acting on the antioxidative defense system.
Keywords
Rhus verniciflua Stokes; neuroprotection; primary cultures of rat cortical cells; glutamate; antioxidant activity;
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1 Kim, Y.C., Kim, S.R., Markelonis, G.J., and Oh, T.H., Ginsenosides Rb1 and Rg3 protect cultured rat cortical cells from glutamate-induced neurodegeneration. J. Neurosci. Res. 53, 426-432 (1998)   DOI   ScienceOn
2 Flohe, L. and Gunzler, W.A., Assays of glutathione peroxidase. Methods Enzymol. 105, 114-121 (1984)   DOI
3 Heintz, N. and Zoghbi, H.Y., Insights from mouse models into the molecular basis of neurodegeneration. Annu. Rev. Physiol. 62, 779-802 (2000)   DOI   ScienceOn
4 Jung, C.H., Jun, C.Y., Lee, S., Park, C.H., Cho, K., and Ko, S.G., Rhus verniciflua Stokes extract: Radical scavenging activities and protective effects on $H_2O_2-induced$ cytotoxicity in macrophage raw 264.7 cell lines. Biol. Pharm. Bull. 29, 1603-1607 (2006)   DOI   ScienceOn
5 McCord, J.M. and Fridovich, I., The utility of superoxide dismutase in studying free radical reactions. . Radicals generated by interaction of sulfite, dimethyl sulfoxide, and oxygen. J. Biol. Chem. 25, 6056-6063 (1969)
6 Trist, D.G., Excitatory amino acid agonists and antagonists: pharmacology and therapeutic applications. Pharm. Acta. Helv. 74, 221-229 (2000)   DOI   ScienceOn
7 Goodman, Y. and Mattson, M.P., Selected forms of b-amyloid precursor protein protect hippocampal neurons against amyloid b-peptide induced oxidative injury. Exp. Neurol. 128, 1-12 (1994)   DOI   ScienceOn
8 Lu, S.C., Regulation of hepatic glutathione synthesis: current concepts and controversies. FASEB J. 13, 1169-1183 (1999)   DOI   PUBMED
9 Mates, J.M. and Sanchez-Jimenez, F.M., Role of reactive oxygen species in apoptosis: implications for cancer therapy. IJBCB 32, 157-170 (2000)
10 Muir, K.W. and Lees, K.R., Clinical experience with excitatory amino acid antagonist drugs. Stroke 26, 503-513 (1995)   DOI   PUBMED   ScienceOn
11 Middleton, Jr.E. and Kandaswani, C., Effects of flavonoids on immune and inflammatory cell functions. Biochem. Pharmacol. 43, 1167-1179 (1992)   DOI   ScienceOn
12 Meldrum, B.S., Implications for neuroprotective treatments. Prog. Brain. Res. 135, 487-495 (2002)   DOI
13 Choi, D.W., Glutamate neurotoxicity and disease of the nervous system. Neuron 1, 623-634 (1988)   DOI   ScienceOn
14 Coyle, J.T. and Puttfarcken P., Oxidative stress, glutamate and neurodegenerative disorders. Science 262, 689-659 (1993)   DOI
15 Choi, D.W., Ionic dependence of glutamate neurotoxicity. J. Neurosci 7, 369-379 (1987)   DOI   PUBMED
16 Tietz, F., Enzymatic method of quantitative determination of nanogram amounts of total and oxidized glutathione. Anal. Biochem. 27, 502- 522 (1969)   DOI   ScienceOn
17 Chase, T.N. and Oh, J.D., Striatal mechanisms and pathogenesis of parkinsonian signs and motor complications. Ann. Neurol. 47, 7-14 (2000)
18 Park, K.Y., Jung, G.O., Lee, K.T., Choi, J., Choi, M.Y., Kim, G.T., Jung, H.J., and Park, H.J., Antimutagenic activity of flavonoids from the heartwood of Rhus verniflua. J. Ethnopharmacol. 90, 73-79 (2004)   DOI   ScienceOn
19 Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Goeke, N.M., Olson, B.J., and Klenk, D.C., Measurement of protein using bicinchoninic acid. Anal. Biochem. 150, 76-85 (1985)   DOI   ScienceOn
20 Kim, T.J., Korea Resource Plants, vol II. Seoul University Press, Seoul, Korea, 292-297, 1996
21 Choi, D.W. and Rothman, S.M., The role of glutamate neurotoxicity in hypoxic-ischemic neuronal death. Annu. Rev. Neurosci. 13, 171-182 (1990)   DOI   ScienceOn
22 Carlberg, I. and Mannervik, B., Purification and characterization of the flavoenzyme glutathione reductase from rat liver. J. Biol. Chem. 25, 5475-5480 (1975)
23 Albright, T.D., Jessell, T.M., Kandel, E.R., and Poster, M.I., Neural science: a century of progress and the mysteries that remain. Cell 18, 1-55 (2000)   DOI   ScienceOn
24 Choi, J., Yoon, B.J., Han, Y.N., Lee, K.T., Ha, J., Jung, H.J., and Park, H.J., Antirhematoid arthritis effect of Rhus verniciflua and of the active component, sulfuretin. Planta Med. 69, 899-904 (2003)   DOI   ScienceOn
25 Lipton, S.A. and Rosenberg, P.A., Excitatory amino acids as a final common pathway for neurologic disorders. N. Engl. J. Med. 330, 613- 622 (1994)   DOI   ScienceOn
26 Lee, J.C., Lim, K.T., and Jang, Y.S., Identification of Rhus verniciflua Stokes compounds that exhibit free radical scavenging and antiapoptotic properties. Biochim. Biophys. Acta. 1570, 181-91 (2002)   DOI   PUBMED   ScienceOn
27 Lim, K.T., Lee, J.C., Jung, H.Y., and Jo, S.K., Effects of Rhus verniciflua Stokes (RVS) on cell-associated detoxificant enzymes, and glucose oxidase-medicated toxicity in cultured mouse hepatocytes. Kor. J. Toxicol. Pub. Health 16, 125-131 (2000)
28 Lee, J.M., Zipfel, G.J., and Choi, D.W., The changing landscape of ischemic brain injury mechanisms. Nature (London) 399, 7-14 (1999)
29 Park, B.C., Lee, Y.S., Park, H.J., Kwak, M.K., Yoo, B.K., Kim, J.Y., and Kim, J.A., Protective effects of fustin, a flavonoid from Rhus verniciflua Stokes, on 6-hydroxydopamine-induced neuronal cell death. Exp. Mol. Med. 39, 316-326 (2007)   DOI   ScienceOn