Browse > Article

Analgesic and Anti-inflammatory Activity of Resina Pini  

Seo, Young-A (College of Pharmacy, Catholic University of Daegu)
Suk, Kui-Duk (College of Pharmacy, Catholic University of Daegu)
Publication Information
Natural Product Sciences / v.13, no.4, 2007 , pp. 347-354 More about this Journal
Abstract
In this study, we investigated the potential of Resina Pini (RP) for anti-inflammatory and analgesic agents to treat inflammatory diseases such as gingivitis and periodontitis. Crude RP (RP1), recrystallized RP (RP2), and Ramus Mori Albae-treated RP (RP3), plus their respective water extracts (RP1-WE, RP2-WE and RP3-WE) were prepared for in vitro and in vivo tests. We couldn't find any signs of heavy metals pollution in all the RP samples. RP2-WE exhibited the highest viability of human gingival fibroblasts (HGF) and the strongest scavenging activity on superoxide anion. RP1, RP2 and RP3, RP2 showed potent scavenging activity on DPPH free radical. RP2-WE displayed a stronger inhibition on hyaluronidase (HAase) activity and RP3 also displayed potent HAase inhibition. RP2-WE, RP3-WE, RP3 and RP2 were reduced admirably the production of $PGE_2$ in HGF. In addition, RP2-WE and RP3-WE exhibited potent inhibitory activities on arachidonic acid-induced ear edema in mouse. Moreover, RP-2 prevented completely acetic acid-induced writhing by 100.0% and RP1, RP3, RP1-WE and RP2-WE also exhibited excellent protective activities against writhing. While aminopyrine, the positive control, showed 76.9% analgesic effect at the same dose. Taken together, these results suggest that recrystallized aqueous extract of Resina Pini could be a promising drug for the treatment of periodontal diseases.
Keywords
Resina Pini; analgesic activity; anti-inflammatory; hyaluronidase; $PGE_2$; cytotoxicity;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Offenbacher, S., Odle, B.M., and Van Dyke, T.E., The use of crevicular fluid prostaglandin $E_2$ levels as a predictor of periodontal attachment loss. J. Periodont Res. 21, 101-112 (1986)   DOI
2 Rubinstein, L.V., Shemaker, R.H., Paul, K.D., Simon, R.M., Tosini, S., Skehan, P., Scudiero, D.A., Monks, A., and Boyd, M.R., Comparison of in vitro anticancer drug-screening data general with a Tetrazolium assay versus a protein assay against a diverse panel of human tumor cell lines. J. Natl. Cancer Inst. 82, 1113 (1990)
3 Saito, S., Saito, M., Ngan, P., Lanese, R., Shanfeld, J., and Davidovitch, Z., Effects of parathyroid hormone and cytokines on prostaglandin E synthesis and bone resorption by human periodontal ligament fibroblasts. Arch. Oral. Biol. 35, 845-855 (1990)   DOI   ScienceOn
4 Silva, J., Abebe, W., Sousa, S.M., Duarte, V.G., Machado, M.I., and Matos, F.J., Analgesic and anti-inflammatory effects of essential oils of Eucalyptus. J. Ethnopharmacol. 89, 277-283 (2003)   DOI   ScienceOn
5 Lorke, D., A new approach to practical acute toxicity testing, Arch. Toxicol. 54, 275-287 (1983)   DOI
6 Mandel, I.D.,.Antimicrobial mouthrinses: overview and update. J. Am. Dent. Assoc, 125, 2S-10S (1994)
7 Namba, T., The encyclopedia of Wakan-Yaku. Vol. , Hoikusa 153-154, 191-194 (1980)
8 Koster, R., Anderson, M., and De Beer, E.J., Acetic acids for analgesic screening. Fed. Proc. 18, 412 (1959)
9 Tung, J.S., Mark, G.E., and Hollis, G.F., A microplate assay for hyaluronidase and hyaluronidase inhibitors. Anal. Biochem. 223, 149- 152 (1994)   DOI   ScienceOn
10 Forrester, J.V. and Balazs, E.A., Inhibition of phagocytosis by high molecular hyaluronate. Immunol. 40, 435-446 (1990)
11 Marklund, S. and Marlund, G., Involvement of the superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide discutase. J. Biochem. 47, 469-474 (1974)
12 Yun, S.O., Fine tree and naturopathy. Academy, Seoul, 67-125, 1997
13 Cho, K.M. and Suk, K.D., The inhibitory action of free radical and arachidonic acid production and cytotoxic effects of Pini Resina. Pharm. Res. 46, 348-351 (2002)
14 Kim, H.K., Namgoong, S.Y., and Kim, H.P. Antiinflammatory activity of flavonoids; Mice ear edema inhibition. Arch. Pharm. Res. 16, 18-24. (1993)   DOI
15 Bacca, L.A., Leusch, M., Lanzalaco, A.C., Macksood D., Bouwsma O.J., Shaffer J.B., Howard-Nordan K.S., Knippenberg S.H., Kreutzjans M.K., Miller J.M., Poore C.L., Sunberg R.J., Vastola K.A. Becus M., Bartizek R.D., Block R.P., Briner W.W., and White D.J., A comparison of intraoral antimicrobial effects of stabilized stannous fluoride dentifrice, baking soda/peroxide dentifrice, conventional NaF dentifrice and essential oil mouthrinse. J. Clin. Dent. 8, 54-61 (1997)   PUBMED
16 Scheie, A.A., Modes of action of currently known chemical anti-plaque agents other than chlorhexidine. J. Dent. Res. 68, 1609-1616 (1989)
17 Schwach-Abdellaoui, K., Loup, P.J., Vivien-Castioni, N., Mombelli, A., Baehni, P., Barr, J., Heller, J., and Gurny, R., Bioerodible injectable poly (ortho ester) for tetracycline controlled delivery to periodontal pockets: preliminary trial in humans. AAPS Pharm. Sci., 4, 20 (2002)
18 Harvey, A., Strategies for discovering drugs from previously unexplored natural products. Drug Discov. Today 5(7), 294-300 (2000)   DOI   ScienceOn
19 Mogil, J.S., Kest B., Sadowsi B., and Belknap J.K., Differential genetic mediation of sensitivity to morphine in genetic models of opiate antinociception: Influence if nociceptive assay. J. Pharm. Exp. Ther. 276, 532-544 (1996)
20 Yuk, C.S., Coloured medical plants of Korea. Academy, Seoul, 175, 1989
21 Tonelli, G., Thiabault, L., and Finglor, J., A bioassay for the concomitant assessment of the antiphiogistic and thymolytic activities of topically applied corticoids. Endocrinol. 77, 625-634 (1965)   DOI   ScienceOn
22 Seo, Y.A., Choi, N.J., and Suk, K.D., Inhibitory Effects of Resina Pini on the Growth and Glucosyltransferase activity of Streptococcus mutans. Nat. Prod. Sci. 11, 27-32 (2005)   과학기술학회마을
23 Song, H.K. and Kim, J.K., Essential oil components of leaves and resins from Pinus densiflora and Pinus koraiensis. Mokchae Konghak 22(3), 55-67 (1994)
24 Hatano, T. Edamatsu, R., Hiramatsu, M., Mori, A., Fujita Y.,Yasuhara T., Yoshida T., and Okuda T., Effects of the interaction of tannins with coexisting substances. .; Effects of tannins and related polyphenols on superoxid anion radical and on 1,1-diphenyl-2-picrylhydrazyl radical. Chem. Pharm. Bull. 37, 2016 (1989)