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The Anti-hyperlipidemic Effect and Constituents of the 19${\alpha}$-Hydroxyursane-type Triterpenoid fraction Obtained from the Leaves of Rusus crataegifolius  

Nam, Jung-Hwan (Department of Botanical Resources, Sangji University)
Jung, Hyun-Ju (College of Pharmacy, Wonkwang University)
Tapondjou, Leon Azefack (Department of Chemistry, Faculty of Science, University of Dschang)
Lee, Kyung-Tae (College of Pharmacy, Kyung-Hee University)
Choi, Jong-Won (College of Pharmacy, Kyungsung University)
Kim, Won-Bae (National Alpine Agriculture Institute, Rural Development Administration)
Park, Hee-Juhn (Department of Botanical Resources, Sangji University)
Publication Information
Natural Product Sciences / v.13, no.2, 2007 , pp. 152-159 More about this Journal
Abstract
To demonstrate anti-hyperlipidemic activity of the 19${\alpha}$-hydroxyursane-type triterpenoid (19${\alpha}$-HUT)-rich fraction, this fraction was prepared from the extract of Rubus crataegifolius leaves. This fraction was found to have anti-hyperlipidemic effect in a high fat diet-induced rat model from the observation of reduction of abdominal fat pad weights, atherogenic index and hypercholesterolemia at 30 and 60 mg/kg (p.o.) The 19${\alpha}$-HUT fraction was subjected to SiO$_2$, ODS, and/or Sephadex LH-20 column chromatography to yield a new triterpenoid (1) called pomolic acid ester along with nine known triterpenoids which are all 19${\alpha}$-HUTs: euscaphic acid (2), tormentic acid (3), 23-hydroxytormentic acid (4), kaji-ichigoside F$_1$ (5), rosamultin (6), niga-ichigosides F$_1$ (7) and F$_2$ (8), suavissimoside F$_1$ (9) and coreanoside F$_1$ (10). The structure of compound 1 was established as 28-O-formyl-3,19-dihydroxyurs-12-en-28-oic acid on the basis of 2D-NMR spectroscopic data and mass spectrum. Compound 1 was isolated for the first time from natural sources.
Keywords
Rubus crataegifolius; Rosaceae; 19${\alpha}$-hydroxyursane-type triterpenoid; pomolic acid ester; NMR; anti-hyperlipidemic;
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