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Gomisin J with Protective Effect Against t-BHP-Induced Oxidative Damage in HT22 Cells from Schizandra chinensis  

An, Ren-Bo (College of Pharmacy, Wonkwang University,College of Pharmacy, Yanbian University)
Oh, Seung-Hwan (College of Pharmacy, Wonkwang University)
Jeong, Gil-Saeng (College of Pharmacy, Wonkwang University)
Kim, Youn-Chul (College of Pharmacy, Wonkwang University)
Publication Information
Natural Product Sciences / v.12, no.3, 2006 , pp. 134-137 More about this Journal
Abstract
Four lignan compounds including gomisin J (1), schizandrin (2), gomisin A (3), and angeloyl gomisin H (4) have been isolated from the MeOH extract of Schizandra chinensis fruits. The evaluation for protective effect of compounds 1-4 against tert-butyl hydroperoxide (t-BHP)-induced cytotoxicity in hippocampal HT22 cell line was conducted. Compound 1 showed significant protective effect with an $EC_{50}$ value of $43.3{\pm}2.3\;{\mu}M$, whereas compounds 2-4 were inactive. Trolox, one of the well-known antioxidant, used as a positive control, and also showed protective effect with an $EC_{50}$ value of $213.8{\pm}8.4\;{\mu}M$. These results suggest that compound 1 may possess the neuroprotective activity against oxidant-induced cellular injuries.
Keywords
gomisin J; Schizandra chinensis; t-BHP-induced cytotoxicity; HT22 cells;
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