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Quercitrin Gallate Down-regulates Interleukin-6 Expression by Inhibiting Nuclear Factor-kB Activation in Lipopolysaccharide-stimulated Macrophages  

Min, Kyung-Rak (College of Pharmacy & Research Center for Bioresource and Health, Chungbuk National University)
Kim, Byung-Hak (College of Pharmacy & Research Center for Bioresource and Health, Chungbuk National University)
Chang, Yoon-Sook (College of Pharmacy & Research Center for Bioresource and Health, Chungbuk National University)
Kim, Young-Soo (College of Pharmacy & Research Center for Bioresource and Health, Chungbuk National University)
Publication Information
Natural Product Sciences / v.12, no.2, 2006 , pp. 113-117 More about this Journal
Abstract
Quercitrin gallate was previously isolated from Persicaria lapathifolia (Polygonaceae) as an inhibitor of superoxide production. In the present study, quercitrin gallate was found to inhibit interleukin (IL)-6 production in lipopolysaccharide (LPS)-stimulated macrophages RAW 264.7 with an $IC_{50}$ value of $63\;{\mu}M$. Furthermore, quercitrin gallate attenuated LPS-induced synthesis of IL-6 transcript but also inhibited LPS-induced IL-6 promoter activity, indicating that the compound could down-regulate IL-6 expression at the transcription level. Since nuclear factor (NF)-kB has been shown to play a key role in LPS-inducible IL-6 expression, an effect of quercitrin gallate on LPS-induced NF-kB activation was further analyzed. Quercitrin gallate exhibited a dosedependent inhibitory effect on LPS-induced nuclear translocation of NF-kB without affecting inhibitory kB (IkB) degradation, and subsequently inhibited LPS-induced NF-kB transcriptional activity in macrophages RAW 264.7. Taken together, quercitrin gallate down-regulated LPS-induced IL-6 expression by inhibiting NF-kB activation, which could provide a pharmacological potential of the compound in IL-6-related immune and inflammatory diseases.
Keywords
Quercitrin gallate; IL-6 expression; NF-kB activation; macrophages RAW 264.7;
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