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Suppression of Cyclooxygenase-2 Expression in Colonic Epithelial Cells by Ilekudinol B Isolated from Weigela subsessilis  

Park, Hye-Jung (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University)
Choi, Yeon-A (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University)
Tae, Jin (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University)
Kang, Chon-Sik (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University)
Kim, Dae-Ki (Department of Immunolgy, Chonbuk National University Medical School)
Thuong, Phuong Thien (College of Pharmacy, Chungnam National University)
Kim, Young-Ho (College of Pharmacy, Chungnam National University)
Bae, Ki-Hwan (College of Pharmacy, Chungnam National University)
Lee, Young-Mi (Department Of Oriental Pharmacy, Colloge of Pharmacy, Wonkwang University)
Publication Information
Natural Product Sciences / v.12, no.1, 2006 , pp. 38-43 More about this Journal
Abstract
Ilekudinol B is one of the flavonoids isolated from Weigela subsessilis (Caprifoliaceae). In the present study, the suppression effect of ilekudinol B on tumor necrosis factor $(TNF)-{\alpha}-induced$ cyclooxygenase-2 (COX-2) expression was investigated in human colon epithelial cell line HT-29. Interleukin-8 (IL-8) production and prostaglandin $E_2\;(PGE_2)$ secretion was measured by enzyme-linked immunosorbent assay (ELISA). COX-2 and nuclear factor $(NF)-{\kappa}B$ expression were determined by Western blot analysis. Ilekudinol B significantly inhibited $TNF-{\alpha}-induced$ secretion of IL-8 and prostaglandin $E_2\;(PGE_2)$ from the human colon epithelial cell line HT-29 in a concentration-dependent manner. In addition, ilekudinol B remarkably diminished $TNF-{\alpha}-induced$ COX-2 expression and $NF-{\kappa}B$ p65 subunit translocation to the nucleus. In conclusion, our results indicate that ilekudinol B may have anti-inflammatory activity on $TNF-{\alpha}-dependent$ colonic inflammation.
Keywords
Weigela subsessilis; ilekudinol B; Caprifoliaceae; cyclooxygenase-2; human colon epithelial cells;
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