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Enhancement of immunological activity in exo-biopolymer from submerged culture of Lentinus edodes with rice bran  

Kim, Hwa-Young (Erom R&D center)
Han, Jae-Taek (Erom R&D center)
Hong, Seong-Gil (Erom R&D center)
Yang, Sung-Bum (Erom R&D center)
Hwang, Sung-Joo (Erom R&D center)
Shin, Kwang-Soon (Department of Food Science and Biotechnology, Kyonggi University)
Suh, Hyung-Joo (Department of Food and Nutrition, College of Health Sciences, Korea University)
Park, Mi-Hyoun (Erom R&D center)
Publication Information
Natural Product Sciences / v.11, no.3, 2005 , pp. 183-187 More about this Journal
Abstract
The objective of the present study was to determine the possible immune-enhancing effects of a substance extracted from a submerged culture of Lentinus edodes with rice bran (SLRB). According to the results obtained by measuring the in vitro macrophage activity of the exo-biopolymer from SLRB, it appears to exhibit activity similar to that of LPS, and this activity seems to occur in a dose-dependent manner. According to the results obtained by measuring splenocyte proliferation, the exo-biopolymer appears to induce an increase in proliferation of approximately 1.4-fold compared to the control group. We measured the proliferation of bone marrow cells in order to evaluate gut immunity and, according to our results, proliferation was increased to 109% that of the control group, and was similar to that associated with LPS. In order to characterize the enhancement of immunological activity in vivo, we orally administered the exo-biopolymer (25, 50, 250 mg/kg bw) to C3H/He mice, and then measured the macrophage activity, determining that the activity was higher than that of the controls at concentrations of 50 and 250 mg/kg. Therefore, the exo-biopolymer from SLRB can be considered to be a useful a BRM agent, as it clearly allows some protection against immunological diseases.
Keywords
rice bran; Lentinus edodes; immune stimulating activity; BRM (biological response modifiers);
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1 Andrewartha, K.A., Philips, D.R., and Stone, B.A., Solution properties of wheat flour arabinoxylans and enzymatically modified arabinoxylans. Carbohydr. Res. 77, 191-204 (1979)   DOI   ScienceOn
2 Chae. S.Y., Shin, S.H., Bae, M.J., and Park, M.H., Effect of arabinoxylane and PSP on activation of immune cells. J. Korea Soc, Food. Sci, Nutr. 33, 278-286 (2004)   DOI
3 Chihara, G., Immune modulation agents and their mechanism (lentinan, a T cell oriented immunopotentiator). Basel, 19, 409-436 (1985)
4 Morel, F., Doussiere, J., and Vignais, P.V., The superoxide generating oxidase of phagocytic cells. Physiological, molecular and pathological aspects. Eur. J. Biochem. 201, 523-546 (1991)   DOI   ScienceOn
5 Ooi, V.E. and Liu, F., Immunomodulation and anti-cancer activity of polysaccharide-protein complexes. Curr. Med. Chem. 7, 715-729 (2000)   DOI   PUBMED   ScienceOn
6 Ramesh, H.P., Yamaki, K., and Tsushida, T., Effect of fenugreek (Trigonella foenum-graecum L.) galactomannan fractions on phgocytosis in rat macrophages and on proliferation and IgM secretion in HB4C5 cells. Carbohydr. Polym. 50, 79-83 (2002)   DOI   ScienceOn
7 Tubaro, A., Florio, C., Luxich, E., Vertua, R., Loggia, R.D., and Yasumoto, T., Suitability of the MTT-based cytotoxicity assay to detect okadaic acid contamination of mussels. Toxicon. 34, 965-974 (1996)   DOI   ScienceOn
8 William, D.G., MGN-3:Quite possibly the most powerful immune booster known to man. Alternatives Health Conscious Individual 7, 113-120 (1998)
9 Williamson, G., Kroon, P.A., and Faulds, C.B., Hairy plant polysaccharides: a close shave with microbial esterase. Microbiology 144, 2011-2023 (1996)   DOI   ScienceOn
10 James, S.P. and Zeitz, M., Human gastrointestinal mucosal T cells, in Pearay, L.O., Jiri, M., Michael, E.L., Warren, S., Jerry, R.M., and John, B(eds)., Handbook of Mucosal Immunology, Academic Press, London, UK, 1994, pp.275-285
11 Choi, E.M., Lim, T.S., Lee, H.L., and Hwang, J.K., Immune cell stimualting activity of wheat arabinoxylan. Korean J. Food Sci, Technol. 34, 510-517 (2002)
12 Hong, S.G., Development of immunostimulation materials from rice bran. Food Industry and Nutrition. 10, 10-15, (2005)
13 Park, M.H., Oh, K.Y., and Lee, B.W., Anti-cancer activity of Lentinus edodes and Pleurotus astreatus. Korean J. Food Sci. Technol. 30, 702-708 (1998)
14 Lee, B.W. and Park, K.M., Anti-tumor activity of protein-bound polysaccharides extracted from mycelia of Lentinus edodes. Korean J. Food Sci. Technol. 30, 665-671 (1998)
15 Sunderkotter, C; Steinbrink, K., Goebeler, M., Bhardwaj, R., and Sorg, C., Macrophages and angiogenesis. J. Leukoc. Biol. 55, 410-422 (1994)   DOI   PUBMED
16 Ghoneum, M., NK immunorestoration of cancer patient by MGN-3, A modified arabinoxylan rice bran(study of 32 patients followed for up to 4 years). Am. Acad. Anti-Aging Med. 1, 1-10 (1999)
17 Hamuro, G. and Wagner, H., ${\beta}$1,3-glucan-mediated augmentation of alloreactive murine cytotoxic T lymphocytes in vivo. Cancer Res. 38, 3080-3088 (1978)   PUBMED
18 Kim, S.W. and Kim, E.S., Studies on the immunomodulating effect of polysaccharide extracted from Ganoderma lucidum on marcophage. Korean J. Sci. Nutr. 26, 148-153 (1997)
19 Hong, T., Matsumoto, T., Kiyohara, H., and Yamada, H., Enhanced production of hematopoietic growth factors through T cell activation in Peyer's patches by oral administration of Kampo (Japanese herbal) medicine, 'Juzen-Taiho-To'. Phytomedicine. 5, 353-360 (1998)   DOI   ScienceOn
20 Burstone, M.S. and Kalpow, L.S., Cytochemical demostration of acid phosphatase in hematopoietic cells in healthy and in various hematological disorder using azo dye technique. J. Histochem. Cytochem. 12, 805 (1964)
21 Denner, G. and Tucker, D., Antitumor polysaccharide lentinan a T cell adjuvant. J. Natl. Cancer. Inst. 51, 1727-1735 (1973)   DOI   PUBMED
22 Ikekawa, T., Uhehara, N., Maeda, Y., Nakanishi, M., and Fukuoka, F., Anti-tumor activities of aquous extracts of some edible mushrooms. Cancer Res, 29, 734-735 (1969)   PUBMED
23 Tzianabos, A., Wang., J.Y., and Kasper, D.L., Biological chemistry of immunomodulation by zwitterionic polysaccharides. Carbohydr. Res. 338, 2531-2538 (2003)   DOI   ScienceOn
24 Henry, R.J., Pentosan and $(1{\rightarrow}3),(1{\rightarrow}4)-beta$ glucan concentrations in endisperm and whole-grain of wheat, barley, oats and rye. J. Creal Sci. 6, 253-258 (1987)   DOI
25 Page, B., Page, M., and Noel, C., A new fluorometric assay of cytotoxicity measurements in vitro. Int. J. Oncol. 3, 473-476 (1993)
26 Juliano, B.O., Rice bran in rice: Chemistry and technology., 2nd., in Julian, B.O.(ed.), American Association of cereal chemist, Inc., St. Paul MN., 1985, pp. 647
27 Hirokazu, O., Current topics in allergy. J. Trad. Sino. Japanese Med. 15, 11-15 (1994)
28 Takuma, S. and Nobuo, T., Further study of the structure of lentinan, antitumor polysaccharides from Lentinus edodes. Carbohydr. Res. 47, 99-104 (1976)   DOI   ScienceOn
29 Ghoneum, M., Enhancement of human natural killer cell activity by modified arabinoxylane from rice bran(MGN-3). Int. J. Immunotherapy. 14, 89-99 (1998)