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1,1-Diphenyl-2-picrylhydrazyl Radical Scavenging Compounds of Fraxini Cortex  

Kim, Hyun-Chul (College of Pharmacy, Wonkwang University)
An, Ren-Bo (College of Pharmacy, Wonkwang University,College of Pharmacy, Yanbian University)
Jeong, Gil-Saeng (College of Pharmacy, Wonkwang University)
Oh, Seung-Hwan (College of Pharmacy, Wonkwang University)
Kim, Youn-Chul (College of Pharmacy, Wonkwang University)
Publication Information
Natural Product Sciences / v.11, no.3, 2005 , pp. 150-154 More about this Journal
Abstract
The radical scavenging effect of the MeOH extract of Fraxini Cortex on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical was examined. The $CH_2Cl_2$-and n-BuOH-soluble fractions of MeOH extract showed the promising DPPH radical scavenging effects, and further purified by silica gel, Sephadex LH-20 column chromatography, and reversed-phase C-18 MPLC to yield five coumarins, esculetin (1), fraxidin (2), fraxetin (3), fraxidin $8-O-{\beta}-D-glucopyranoside$ (fraxin methyl ether) (5), esculin (6), and a secoiridoid oleuropein (4), and a coumarin-secoiridoid escuside (7). Compounds 1, 3, and 4 showed potent DPPH radical scavenging effects, exhibiting $IC_{50}$ values of 14.68, 9.64, and $22.03\;{\mu}M$, respectively. Compounds 6 and 7 also showed moderate effects with $IC_{50}$ values of 147.79 and $72.73\;{\mu}M$, respectively. L-Ascorbic acid was used as a positive control and exhibited the $IC_{50}$ value of $50.31\;{\mu}M$.
Keywords
Fraxini Cortex; Oleaceae; DPPH; Anti-radical; Coumarin; Oleuropein; Coumarin-secoiridoid;
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