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Studies on The Lypolitic Enzymes of Carum Roxburgllianum Seed Meal

Mahmud, Shahid (Applied Chemistry Research Centre, PCSIR Laboratories Complex)
Waheed, Amran (Applied Chemistry Research Centre, PCSIR Laboratories Complex)
Khanum, Razia (Applied Chemistry Research Centre, PCSIR Laboratories Complex)

Publication Information

Natural Product Sciences / v.10, no.6, 2004 , pp. 302-305 More about this Journal

Abstract

The lipase and phospholipase activities of meals of resting seeds of C. roxburghianum were studied at different temperatures, solvents and pH. Both the enzymes showed the maximum activities at $40^{\circ}C$ and in n-heptane used as solvent. However, lipase showed maximum activities at two different pH, one at pH 5 (acidic) and other at pH 8 (alkaline) whereas phospholipase showed only one pH optimum at pH 8. During the course of germination, the lipase showed an increase whereas reverse was the case with phospholipase.

Keywords

Lipase; phospholipase; enzymes; carum;

Citations & Related Records

Times Cited By SCOPUS : 0

Reference

1	Javed, M.A, Waheed, A and Akhtar, N., Studies of Lipase and phospholipase from the meal of Citrullus colocyuthis of the family Cucurbidaceae, Proc. Pak. Ace Sci. 36,47-51 (1999)
2	Waheed, A, Mahmud, S., Javed, M.A and Saleem, M. Activity lipase and phospholipase extracted from the seeds of Nicotiana rustica of family Solanaceue, Proc. Pak. Acad. Sci. 39, 74-78 (2002)
3	Banu, C., and Serban, L. Enzymatic changes on dehydrated products, Lipase activity in some oleaginous seeds Individual. Ailment (Bucharest) 21, 367-369 (1970)
4	Zaka, S., Akhtar, M.W and Khan S.A. Phospholipase acy hydrolase and Triglyceride acyl-hydrolase actlVltIeS in the developing seeds of Cassia species, Pak. J. Sci. Ind. Res., 32, 27-32 (1989)
5	Waheed, A, Mahmud, S., Saleem, M., Yamin, M. and Naeem M. Lipid Studies of Carum roxburghianum Seeds. Natural Product Sciences, (Korea) 9, 200-203 (2003)
6	Kausar, N. and Akhtar, M.W, Isolation and Characterization of Hibiscus cannabinus seed lipase. Pak. J. Biochem. 12, 58-64 (1979)
7	Akhtar, M.W, Parveen, H., Kausar, S. and Chughtai, M.LD., Lipase activity in plant seeds, Pak. J. Biochem. 8, 77-82 (1975)
8	Guven, K.C., Bergisadi, N. and Guier, E. A modification of duncombes method and its application to the lipolytic assay of heparin. Fetter Seifen Anstrichmittel, 4, 152-154 (1989)
9	Blain, J.A, Akhtar, M.W and Patterson, J.D.E. Enzyme activity in organic solvents. Pak. J. Biochem. 9,41-45 (1976)
10	Dry, R.L. Acid Lipase of Castor bean. Lipids 4, 177-185 (1969). DOI
11	Chopra, G.L. 'Angisperms', Uniques Publishers Lahore, $9^{th}$ ed., p. 248 (1970)
12	Bhatty, M.K, 'Essential oils of the plant family umbelliferae' (Final Report) PL-480, PCSIR Laboratories, Lahore, Pakistan (1977)
13	Aman, T., Akhtar, M.W and Jamahid, F, Isolation and characterization of Zea mays (Neelum) root alkaline lipase. Sci. Int. Lahore, 192-195 (1989)
14	Kirtikar, KR. and Basu, B.D., 'Indian Medicinal Plants', $2^{nd}$ ed., Vol. II, pp.936-939, Bishen Singh and Mahendra Pal Publishers, 23A, New Connaught Place, Dehra Dun, 24-8001, India (1984)
15	Berner, D.L. and Hammond, E.G. Specificity of lipase from several seeds lipids 5, 572-573 (1972)
16	Ferrigan, M. and Geddes, W.E. Distribution of lipase in the commercial mill products from hard red spring wheat. Cereal Chem. 35, 422-427 (1958)