Browse > Article

A Molecular Switch for the Induction of Resveratrol Biosynthesis in Grapes  

Lee, Mi-Sook (Department of Molecular Biology and Institute of Nanosensor and Biotechnology, Dankook University)
Pyee, Jae-Ho (Department of Molecular Biology and Institute of Nanosensor and Biotechnology, Dankook University)
Publication Information
Natural Product Sciences / v.10, no.5, 2004 , pp. 248-251 More about this Journal
Abstract
Resveratrol has been reported to possess a variety of biological and pharmaceutical activities. Regardless of its beneficial effects on health, the amount of resveratrol in grapes is very low. In order to induce the resveratrol biosynthesis, the promoter region of a genomic fragment encoding the resveratrol synthase was isolated and a molecular switch was identified which provides us with defining biotic or abiotic inducers that transcriptionally up-regulate the gene expression involved in the resveratrol biosynthesis. We could successfully increase the amount of resveratrol in grapes up to 3-fold by using these environmental factors.
Keywords
resveratrol; resveratrol synthase gene; promoter; up-regulate; biotic or abiotic inducers;
Citations & Related Records

Times Cited By SCOPUS : 2
연도 인용수 순위
1 Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the pri nciple of protein-dye binding. Anal. Biochem. 72, 248-254 (1976)   DOI   PUBMED   ScienceOn
2 Fliegmaim, J., Gudmn, S., Schanz, S., Britsch, L., and Schrode, J., Molecular analysis of chalcone and dihydropinosylvm synthase from Scot pine (Pinus sytvestris), and differential regulation of these and related enzyme activities in stressed plants. Plant Mol Biol. 18, 489-503 (1992)   DOI   ScienceOn
3 Langcake, P. and Pryce, R.J., The production of resveratrol by Vitis viniera and other members of the Vitaceae as a response to infection or injury. Physiol Plant Patlot. 9, 77-86 (1976)   DOI
4 Okuda, T. and Yokotsuka, K., Trans-resveratrol concentration in berry skins and wines from grapes grown in Japan. Am. J. Enol. Vitic. 47, 93-99 (1990)
5 Yoshikawa, M., Diverse modes of action of biotic and abiotic phytoalexin elicitors. Nature 275, 546-547 (1978)   DOI
6 Langcake, P. and Pryce, R.J., The production of resveratrol by Ws vinifera and other members of the Vitaceae as a response to infection or injury. Physiol Plant Pathol. 9, 77-86 (1976)   DOI
7 Adrian, M.R, Jeandet, R, Bessis, R., and Jouber, J.M., Inducdon of phytoalexin (resveratrol) synthesis in grapevine leaves treated with aluminium chloride (AlCla). J. Agric. Food Chem. 44, 1979-1981 (1996)   DOI   ScienceOn
8 Jefferson, R.A., Kavanagh, T.A., and Bevan, M.W., GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion mar ker in higher plants. EMBO J. 6, 3901-3907 (1987)
9 Schubert, R., Fischer, R., Hain, R., Schreier, P.H., Bahnweg, G., Emst, D., and Sandermann, H. Jr., An ozone-responsive region of the grapevine resveratrol synthase promoter differs from the basal pathogen-responsive sequence. Plant Mol. Biol. 34, 417-426 (1997)   DOI   ScienceOn
10 Hain, R., Bieseler, B., Kindl, H., Schroder, G., and Stocker, R., Expression of a stilbene synthase gene in Nicotiana tabacum results in synthesis of the phytoalexin resveratrol. PIant Mol. Biol. 15, 325-35 (1990)   DOI   ScienceOn
11 Cantos, E., Espin, J.C., and Tomas-Barberan, F.A., Postharvest induction modeling method using UV irradiation pulses for ob taining resveratrol-enhched table grapes: A new functional fruit? J. Agrc. Food Chem. 49, 5052-5058 (2001)   DOI   ScienceOn
12 Melchior, F. and Kindl, H., Coordinate- and elicitor-dependent expression of stilbene synthase and phenylalanine ammonia-lyase genes in Vitis cv. Optima. Arch. Biochem. Biophys. 288,552-7 (1991)   DOI   ScienceOn
13 Jang, M., Cai, L., Udeani, G.O., Slowing, K.V., Thomas, C.F., Beecher, C.W.W., Fong, H.H.S., Famsworth, N.R., Kinghom, A.D., Mehta, R.G., Moon, R.C., and Pezzuto, J.M., Cancer chemopreventive activity of resveratrol, a natural product derived from grapes. Science 275, 218-220 (1997)   DOI   PUBMED
14 Schoeppner, A. and Kindle, H., Stilbene synthase (Pinosylvine sy nthase) and its induction by ultraviolet light. FEBS Lett. 108, 349-352 (1979)   DOI   ScienceOn
15 Langcake, P. and Pryce, R.J., The production of resveratrol and the viniferins by grapevines in response to ultraviolet irradiation. Phytochemistry 16, 1193-1196 (1977)   DOI   ScienceOn
16 Sparvoli, R, Martin, C., Scienza, A., Gavazzi, G., and Tonelli, C., Cloning and molecular analysis of structural genes involved infla vonoid and stilbene biosynthesis in grape (Vitis vinifera L). PtantMol. Biol. 24, 743-755 (1994)
17 Wiese, W., Vomam, B., Krause, E., and Kindl, H., Structural organization and differential expression of three stilbene synthase genes located on a 13 kb grapevine DNA fragment Plant Mol. Biol. 26, 667-677 (1994)   DOI   ScienceOn
18 Donnelly, L.E., Newton, R., Kennedy, G.E., Fenwick, P.S., Leung, R.H., Ito, K., Russell, R.E., and Barnes, P.J., Anti-inflammatory effects of resveratrol in lung epithelial cells: molecular me chanisms. Am. J. Physiol. Lung Cell Mol. Physiol 287, L774-783 (2004)   DOI   PUBMED   ScienceOn
19 Clough, S.J. and Bent, A.F., Floral dip: a simplified method for Agrobacterium-medated transformation of Arabidopsis thatiana. Plant J. 16, 735-743 (1998)   DOI   ScienceOn
20 Olas, B., Zbikowska, H.M., Wachowicz, B., Krajewski, T., and Buczynski, A., Magnuszewska, A. Inhibitory effect of resveratrol on free radical generation in blood platelets. Acta Biochem. Pol. 46, 961-966 (1999)
21 Emst, D., Schraudner, M., Langebanels, G., and Sandermann, H., Ozone-induced changes of mRNA level of P,-1,3-g1ucanase, chitinase and pathogenesis-related protein in tobacco plants. Ptant Mol. Biol. 20, 673-682 (1992)   DOI   ScienceOn
22 Doyle, J.J. and Doyle, J.L, Isolation of plant DNA from fresh tissue. Focus 12, 13-15 (1990)