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Inhibitory Effect of Ligularia fischeri var. spiciformis and Its Active Component, 3,4-Dicaffeoylquinic Acid on the Hepatic Lipid Peroxidation in Acetaminophen-Treated Rat  

Choi, Jong-Won (College of Pharmacy, Kyungsung University)
Park, Jung-Kwan (College of Pharmacy, Kyungsung University)
Lee, Kyung-Tae (College of Pharmacy, Kyung-Hee University)
Park, Kwang-Kyun (Department of Dentistry, Younsei University)
Kim, Won-Bae (National Alpine Expermental Station, RDA)
Lee, Jin-Ha (Department of Food Manufacturing, Kangwon University)
Jung, Hyun-Ju (Department of Botanical Resources, Sangji University)
Park, Hee-Juhn (Department of Botanical Resources, Sangji University)
Publication Information
Natural Product Sciences / v.10, no.4, 2004 , pp. 182-189 More about this Journal
Abstract
To find the action mechanism of the MeOH extract (LFS) of Ligularia fischeri var. spiciformis herbs (Compositae) and its active component, 3,4-dicaffeoylquinic acid (DCQA) on antihepatotoxicity, the effect was investigated on hepatic lipid perxodation and drug-metabolizing enzyme activities in acetaminophen-treated rat. Pretreatment with 250 mg/kg LFS (p.o.) and 10 mg/kg DCQA (p.o.) significantly decreased hepatic lipid peroxidation caused by acetaminophen injection. Further, LFS and DCQA inhibited hepatic microsomal enzyme activation such as hepatic P-450 cytochrome $b_5$, aniline hydroxylase and aminopyrine N-demethylase, suggesting that the two substances might effectively prevent the metabolic activation or scavenge electrophilic intermediates capable of causing hepatotoxicity. Both LFS and DCQA increased hepatic glutathione content and glutathione reductase activity, indicating that both resultantly prevented hepatotoxicity via antioxidative mechanism. Therefore, it was found that LFS had antihepatotoxicity based on the antioxidative action of DCQA.
Keywords
Ligularia fischeri var. spiciformis; 3,4-dicaffeoylquinic acid; antihepatotoxic; lipid peroxidation;
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Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By SCOPUS : 6
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