Browse > Article

Flavonoid Fraction Purified from Rhus verniciflua Stokes Actively Inhibits Cell Growth Via Induction of Apoptosis in Mouse Tumorigenic Hepatocytes  

Lee, Jeong-Chae (Department of Orthodontics and Institute of Oral Bioscience, Chonbuk National University)
Publication Information
Natural Product Sciences / v.10, no.2, 2004 , pp. 74-79 More about this Journal
Abstract
Dietary flavonoids are currently receiving considerable attention in developing novel cancer-preventive approaches because of their potential capacities to actively induce apoptosis of cancer cells. In our previous report, a flavonoid fraction, which consisted mainly of protocatechuic acid, fustin, fisetin, sulfuretin, and butein and named RCMF (RVS chloroform-methanol fraction), was prepared from a crude acetone extract of Rhus verniciflua Stokes (RVS) that is traditionally used as food additive and herbal medicine. In this study, we evaluated the effects of the RCMF on cell proliferation and apoptosis using SV40-transformed tumorigenic hepatocytes, BNL SV A.8. Tritium uptake assay showing the proliferative capacity of the cells was strongly suppressed in the presence of RCMF. This anti-proliferative effect was further confirmed through trypan blue exclusion. RCMF-mediated suppression of cell growth was verified to be apoptotic, based on the increase in DNA fragmentation, low fluorescence intensity in nuclei after propidium iodide staining, and the appearance of DNA laddering. Collectively, this study demonstrated that RCMF can be approached as a potential agent that is capable of significantly inhibiting cell growth of hepatic cancer cells.
Keywords
Rhus verniciflua Stokes; Flavonoids; Hepatocytes; Growth inhibition; Apoptosis;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Caltagirone, S., Rossi, c., Poggi, A., Ranelletti, F.O., Natali, P.G., Brunetti, M., Aiello, F.B., and Piantelli, M., Flavonoids apigenin and quercetin inhibit melanoma growth and metastatic potential. Int. J. Cancer 87, 595-600 (2000)   DOI   ScienceOn
2 Christou, L., Hatzimichael, E., Chaidos, A., Tsiara, S., and Bourantas, K.L., Treatment of plasma cell leukemia with vincristine, liposomal doxorubicin and dexamethasone. Eur. J. Haematol. 67,51-53 (2001)   DOI   ScienceOn
3 Duarte, J., Perez-Vizcaino, F., Zarzuelo, A., Jimenez, J., and Tamargo, J., Vasodilator effects of quercetin in isolated rat vascular smooth muscle. Eur. J. Pharmacol. 239, 1-7 (1993)   DOI   PUBMED   ScienceOn
4 Kitts, D.D., and Lim, K.T., Antitumorigenic and cytotoxic properties of an ethanol extract derived from Rhus verniciflua Stokes (RVS). J. Toxicol. Env. Health 64, 357-371 (2001)   DOI   ScienceOn
5 Lee, J.C., Jung, H.Y, and Lim, K.T., Effects of Rhus verniciflua stokes (RVS) on the plasma level of cholesterol and tumor growth in mouse. J. Toxicol. Pub. Health 15,169-175 (1999)
6 Lee, J.C., Kim, J., and Jang, Y.S., Ethanoleluted extract of Rhus verniciflua Stokes inhibits cell growth and induces apoptosis in human lymphoma cells. J. Biochem. Mol. Biol. 36, 337-343 (2003)   DOI
7 Lee, J.C., Kim, J., Park, J.K, Chung, G.H., and Jang, Y.S., The anti oxidant, rather than pro oxidant, activities of quercetin on nornal cells: Quercetin protects mouse thymocytes from glucose oxidase mediated apoptosis. Exp. Cell Res. 291, 386-397 (2003)   DOI   ScienceOn
8 Mukherjee, A,K., Basu, S., Sarkar, N., and Ghosh, A.C., Advances in cancer therapy with plant based natural products. Curr. Med. Chem. 8, 1467-1486 (2001)   DOI   ScienceOn
9 Paschka, A.G., Butler, R., and Young, C.Y.F., Induction of apoptosis in prostate cancer cell lines by the green tea component, (-)-epigallocatechin-3-gallate. Cancer Lett. 130, 1-7 (1998)   DOI   PUBMED   ScienceOn
10 Pezutto, J.M., Plant derived anticancer agents. Biochem Pharmacol. 53, 121-133 (1997)   DOI   PUBMED   ScienceOn
11 Plaumann, B., Fritsche, M., Rimpler, H., Brandner, G., and Hess, R.D., Flavonoids activate wild type p53. Oncogene 13, 1605-1614 (1996)
12 Formica, J.V., and Regelson, W., Review of the biology of quercetin and related bioflavonoids. Food Chem. Toxicol. 33, 1061-1080 (1995)   DOI   ScienceOn
13 Wong, W.S., and McLean, A.E., Effects of phenolic antioxidants and flavonoids on DNA synthesis in rat liver, spleen, and testis in vitro. Toxicology 139, 243-253 (1999)   DOI   ScienceOn
14 Gao, Z., Huang, K., Yang, X., and Xu, H., Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi. Biochim. Biophys. Acta 1472, 643-650 (1999)   DOI   ScienceOn
15 Gupta, S., Afaq, P., and Mukhtar, H., Selective growth-inhibitory, cell-cycle deregulatory and apoptotic response of apigenin in nonnal versus human prostate carcinoma cells. Biochem. Biophys. Res. Commun. 287, 914-920 (2001)   DOI   ScienceOn
16 Miranda, C.L., Stevens, J.F., Helmrich, A., Henderson, M.C., Rodriguez, R.J., Yang, Y.H., Deinzer, M.L., Barnes, D.W, and Buhler, D.R., Antiproliferative and cytotoxic effects of prenylated flavonoids from hops (Humulus lupulus) in human cancer cell lines. Food chem. Toxicol. 37, 271-285 (1999)   DOI   ScienceOn
17 Yoshioka, K., Deng, T., Cavigelli, M., and Karin, M. Antitumor promotion by phenolic antioxidants: inhibition of AP-1 activity through induction of Fra expression. Proc. Natl. Acad. Sci. USA. 92, 4972-4976 (1995)   DOI   ScienceOn
18 Smets, L.A., Programmed cell death (apoptosis) and response to anti cancer drugs. Anti Cancer Drugs 5, 3-9 (1994)   PUBMED
19 Yang, E.B., Zhang, K., Cheng, L.Y, and Mack, P., Butein, a specific protein tyrosine kinase inhibitor. Biochem. Biophys. Res. Commun. 245, 435-438 (1998)   DOI   ScienceOn
20 Zhang, H.L., Nagatsu, A., Okuyama, H., Mizukami, H., and Sakakibara, J., Sesquiterpene glycosides from cotton oil cake. Phytochemistry 48, 665-668 (1998)   DOI   ScienceOn
21 Lee, J.C., Kim, J., Lim, K.T., Yang, M.S., and Jang, Y.S., Ethanol eluted extract of Rhus verniciflua Stokes showed both antioxidant and cytotoxic effects on mouse thymocytes depending on the dose and time of the treatment. J. Biochem. Mol. Biol. 34, 250-258 (2001)
22 Gamet Payrastre, L., Manenti, S., Gratacap, M.P., Tulliez, J., Chap, H., and Payrastre, B., Flavonoids and the inhibition of PKC and PI3 kinase. Gen. Pharmacol. 32, 279-286 (1999)   DOI   ScienceOn
23 Kim, l.W, Shin, D.H., and Baek, N.l., Identification of antioxidative components from ethanol extract of Rhus verniciflua Stokes. Kor. J. Food Sci. Technol. 31, 1654-1660 (1999)
24 Hong, D.H., Han, S.B., Lee, C.W, Park, S.H., Jeon, Y.J., Kim, M.J., Kwak, S.S., and Kim, H.M., Cytotoxicity of urushiols isolated from sap of Korean lacquer tree (Rhus verniciflua Stokes). Arch. Pharmacol. Res. 22, 638-641 (1999)   DOI   ScienceOn
25 Lee, J.C., Lim, K.T., and Jang, Y.S., Identification of Rhus vemiciflua Stokes compounds that exhibit free radical scavenging and anti apoptotic properties. Biochim. Biophys. Acta 1570,181-191 (2002)   DOI   ScienceOn
26 Whitmarsh, A.J., and Davis, R.J. Transcription factor AP-1 regulation by mitogen-activated protein kinase signal transduction pathways. J. Mol. Med. 74, 589-607(1996)   DOI   ScienceOn
27 Jung, N.C., Biological activity of urushiol and flavonoids from Lac tree (Rhus verniciflua Stokes). Ph.D. thesis, Chonnam National University, Kwang-ju, South Korea (1998)