Browse > Article

Inhibition of Porcine Pancreatic Elastase (PPE) by Korean Mistletoe (Viscum album var.coloratum) Fractions  

Lyu, Su-Yun (School of Pharmaceutical Sciences, Boots Science Building, University of Nottingham)
Moon, You-Sun (College of Natural Sciences, Seoul Womes University)
Kwon, Young-Ju (College of Natural Sciences, Seoul Womes University)
Joo, Hye-Jin (College of Natural Sciences, Seoul Womes University)
Park, Won-Bong (College of Natural Sciences, Seoul Womes University)
Publication Information
Natural Product Sciences / v.9, no.4, 2003 , pp. 278-285 More about this Journal
Abstract
The serine proteases such as human leukocyte elastase (HLE) and porcine pancreatic elastase (PPE) are classified in the chymotrypsin family, and possibly the most destructive enzymes having the ability to degrade virtually all of the connective components in the body. In the present study, the extracts of water and methanol of Korean mistletoe (Viscum album var. coloratum) inhibited significantly the PPE activity. The fractions eluated on Amberlite XAD-2 from methanol extract were further purified on the repeated $SiO_2$ column chromatography and the fractions A, B and C were eluated. The fractions A, B and C at 3 mg/ml inhibited significantly the PPE activity up to 66%, 95% and 85%, respectively. In conclusion, the fraction A assumed as lignans or phenylpropanes, and fraction B and C assumed as triterpenoids showed the PPE inhibitory effects on the PPE and that these compounds in mistletoe may be used for treatment of pathological processes such as age-dependent tissue loss or inflammation.
Keywords
Mistletoe; Elastase; PPE; Inhibition;
Citations & Related Records

Times Cited By SCOPUS : 1
연도 인용수 순위
1 Yager D .R, Chen S. M., Ward S. I., Olutoye O. O., Diegelmann R. F, and Cohen I. K., Ability of chronic wound fluids to degrade peptide growth factors is associated with increased levels of elastase activity and diminished levels of proteinase inhibitors. Wound Repair and Regeneration 5, 2332 (1997)
2 Wagner H, Feil B, Seligmann O, Petricic J, and Kalogjera Z, Phenylpropanones and lignans of Viscum album cardioactive drugs. V. Planta Medica, 2, 102-104 (1986)
3 Cowan K. N., Heilbut A., Humpl T.,Lam C; Ito S., and Rabinovitch M., Complete reversal of fatal pulmonary hypertension in rats by a serine elastase inhibitor. Nat. Med., 6, 698702 (2000)   DOI   ScienceOn
4 Cregge R. J., Durham S. L., Farr R. A., Gallion S. L., Hare C .M., Hoffman R. V; Janusz M. J., Kim H. O., Koehl J. R, Mehdi S., Metz W. A., Peet N. P, Pelton J. P, Schreuder H. A., Sunder S., and Tardif C, Inhibition of human neutrophil elastase. 4. Design, synthesis, X-ray crystallographic analysis, and structure-activity relationships for a series of P2-modified, orally active peptidyl pentafluoroethyl ketones. J. Med. Chem., 41,2461-2480 (1998)   DOI   ScienceOn
5 Gerard S., Dive G., Clamot B., Touillaux R, and Marchand-Brynaert J., Synthesis, hydrolysis, biochemical and theoretical evaluation of 1,4-bis(alkoxycarbonyl)azetidin-2-ones as potential elastase inhibitors, Tetrahedron, 58, 2423-2433 (2002b)   DOI   ScienceOn
6 Kong D. Y, Li H. T.,and Luo S. Q., Chemical constituents of Viscum coloratum VII. Isolation and structure of viscumneoside VII. Yaoxue Xuebao, 258, 608-611 (1990)
7 Lyu, S. Y., Park, W. B., Choi, K. H., and Kim, W. H., Involvement of caspase-3 in apoptosis induced by VlScum album var. coloratum agglutinin in HL-60 cells. Biosci. Biotechnol. Biochem., 65, 534-541 (2001)   DOI   ScienceOn
8 Siedle B., Cisielski S., Murillo R, Loser B., Castro V, Klaas C. A., Hucke O., Labahn A., Melzig M. F., and Merfort I., Sesquiterpene lactones as inhibitors of human neutrophil elastase, Bioorg. Med. Chem., 10, 2855-2861 (2002)   DOI   ScienceOn
9 Wiedow O., Wiese F., Streit V.. Kalm C, and Christophers E., Lesional elastase activity in psoriasis contact dermatitis and atopic dermatitis. J. Invest. Dermatol.. 99, 306-309 (1992)   DOI   ScienceOn
10 Khorramizadeh M. and Tredget E., Aging differentially modulates the expression of collagen and collagenase in dermal fibroblasts. Mol Cell Biochem,194, 99108 (1999)
11 Rinderknecht H.. Pancreatic secretory enzymes. In: Go V. L. W., Dimagno E. P., Gardner J. D., Lebenthal E., and Reber H. A., Scheele GA (eds.) The pancreas biology, pathobiology and disease. 2nd ed. New York, Raven Press, 225-7 (1993)
12 Cabaret D., Gonzales M. G., Wakselman M., Adediran S. A., and Pratt R. F, Synthesis, hydrolysis, and evaluation of 3-acylamino3,4- dihydro-2-oxo-2H-l,3-benzoxazinecarboxylic acids and linear azadepsipeptides as potential substrates/inhibitors of $\beta$-lactamrecognizing enzymes. Eur. J. Org. Chem., 1, 141-150 (2001)
13 Grinell F. and Zhu M., Identification of neutrophil elastase as the proteinase in bum wound fluid responsible for degradation of fibronectin. J. Invest. Dermatol. 103, 155161 (1994)   DOI   ScienceOn
14 Rai R and Katzenellenbogen J.A., Effect of conformational mobility and hydrogen-bonding interactions on the selectivity of some guanidinoaryl-substituted mechanism-based inhibitors of trypsinlike serine proteases J. Med. Chem. 35,4297-4305 (1992)   DOI   PUBMED
15 Robert L. and Robert A. M., Recent advances in elastase inhibitors. Curr. Opin. Ther. Pat. 2,573581 (1992)
16 Tiefenbacher C. P., Ebert M., Niroomand F., Batkai S., Tillmanns H., Zimmermann R., and Kubler W., Inhibition of elastase improves myocardial function after repetitive ischaemia and myocardial infarction in the rat heart. Pflugers Arch., 433, 563570 (1997)   DOI   ScienceOn
17 Vogelmeier C., Hubbard R. C., Fells G. A, Schnebli H. P., Thompson R. C., Fritz H., and Crystal R.G., Anti-neutrophil elastase defense of the normal human respiratory epithelial surface provided by the secretory leukoprotease inhibitor. J. Clin. Invest., 87,482488 (1991)   DOI
18 Gerard S., Nollet G., Put J. V.. and Marchand-Brynaert J., 1Alkoxycarbonyl- 3-halogenoazetidin-2-ones as elastase (PPE) inhibitors, Bioorg. Med. Chem., 10, 3955-3964 (2002a)   DOI   ScienceOn
19 Lyu, S. Y., Choi S. H., and Park W. B., Korean mistletoe lectin-induced apoptosis in hepatocarcinoma cells is associated with inhibition of telomerase via mitochondrial controlled pathway independent of p53. Arch. Pharm. Res., 25, 93-101 (2002)   DOI   ScienceOn
20 Lee J. K., Zaidi S. H., Liu P., Dawood F, Cheah A. Y, Wen W. H., Saiki Y., and Rabinovitch M., A serine elastase inhibitor reduces inflammation and fibrosis and preserves cardiac function after experimentally-induced murine myocarditis. Nat. Med., 4, 13831391 (1998)   DOI   ScienceOn
21 Travis J., Mechanism for alteration of the proteinaseproteinase inhibitor balance in tissue. In: Katunuma N., Suzuki K., Travis J., and Fritz H. (eds), Biological functions of proteases and inhibitors, Japan Scientific Societies Press, Tokyo, pp. 221229 (1994)
22 Fukunaga T., Kajikawa I., Nishiya K., Takeya K. and Itokawa H., Studies on the constituents of the Japanase misteltoe Viscum album L. var. coloratum Ohwi grown on different trees and their antimicrobial and hypotensive properties. Chem. Pharm. Bull., 37, 1543-1546 (1989)   DOI   ScienceOn
23 Pochet L., Doucet C, Dive G., Wouters J., Masereel B., Reboux Ravaux M., and Pirotte B., Coumarinic derivatives as mechanismbased inhibitors of $\alpha$ -chymotrypsin and human leukocyte elastase. Bioorg. Med. Chem., 8, 1489-1501 (2000)   DOI   PUBMED   ScienceOn
24 Shapiro S., Matrix metalloproteinase degradation of extracellular matrix: biological consequences. Curr. Opin. Cell Biol., 10, 602-608 (1998)   DOI   PUBMED   ScienceOn
25 Ahn W. Y, Analysis of chemical constituents of saccharides and triterpenoids in the Korean native mistletoes. Mokchae Konghak; 24,27-33 (1995)
26 Kerrigan J. E., Walters M. C, Forrester K. J., Crowder J. B.and Christopher L. J, 6-Acylamino-2-[(alkylsulfonyl)oxy]-1H-isoindole1,3- dione mechanism-based inhibitors of human leukocyte elastase. Bioorg. Med. Chem. Lett., 10, 27-30 (2000)   DOI   ScienceOn
27 Homsy R., Pelletier-Lebon P., Robert L, and Homebeck W., Fibroblast elastase(s). In: L Robert and W Homebeck (eds.), Elastin and elastases Vol. 2, CRC Press, pp. 5762 (1989)
28 Scheele G., Bartelt D., and Bieger w., Characterization of human exocrine pancreatic proteins by two-dimensional isoelectric focusing/sodium dodecyl sulfate gel electrophoresis. Gastroenterology, 80,461-73 (1981)
29 Bussing A., Biological and pharmacological properties of viscum album L.; From tissue flask to man. In A. Bussing (eds.), Mistletoe, genus Viscum & other genera, Medicinal and aromatic plants-Industrial profiles. Harwood Academic Publishers, Netherland, pp 45-60 (2000)
30 Kuang S. H. R, Venkataraman R, Tu J., Truong T. M., Chan H. K., and Groutas W. C, Potent inhibition of serine proteases by heterocyclic sulfide derivatives of 1,2,5-thiadiazolidin-3-one 1,1 dioxide. Bioorg. Med. Chem., 8, 1713-1717 (2000)   DOI   ScienceOn
31 Lyu, S. Y., Park, S. M., Choung, B. Y., and Park, W. B., Comparative study of Korean (Viscum album, var. coloratum) and European mistletoes (Viscum album). Arch. Pharm. Res., 23, 592-598 (2000)   DOI   ScienceOn
32 Yamamoto H., Koizumi T., Kaneki T., Hanaoka M., and .Kubo K., Effects of lecithinized superoxide dismutase and a neutrophil elastase inhibitor (ONO-5046) on hyperoxic lung injury in rat. Eur. J. Pharmacol., 409, 179183 (2000)   DOI   ScienceOn
33 Zhang H., Patel S. A., Kandil E., Mueller C. M., Lin Y. Y., and Zenilman M. E., Pancreatic elastase is proven to be a mannosebinding proteinimplications for the systemic response to pancreatitis, Surgery, 133, 678-688 (2003)   DOI   ScienceOn
34 Edwards P. D. and Bemstein P.R, Synthetic inhibitors of elastase. Med. Res. Rev., 14, 127194 (1994)   DOI   ScienceOn