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http://dx.doi.org/10.1186/s41240-019-0134-3

Regulation of adductor muscle growth by the IGF-1/AKT pathway in the triploid Pacific oyster, Crassostrea gigas  

Kim, Eun-Young (Institute of Fisheries Sciences, Pukyong National University)
Choi, Youn Hee (Institute of Fisheries Sciences, Pukyong National University)
Publication Information
Fisheries and Aquatic Sciences / v.22, no.9, 2019 , pp. 19.1-19.10 More about this Journal
Abstract
We investigated the insulin-like growth factor 1 (IGF-1)/AKT signaling pathway involved in muscle formation, growth, and movement in the adductor muscle of triploid Pacific oyster, Crassostrea gigas. Large and small triploid oysters (LTs and STs) cultured under identical conditions were screened, and the signaling pathways of individuals with superior growth were compared and analyzed. mRNA and protein expression levels of actin, troponin, tropomyosin, and myosin, proteins important in muscle formation, were higher in LTs compared with STs. Expression levels of IGF-1, IGF binding protein (IGFBP), and IGFBP complex acid-labile subunit were also higher in LTs compared with STs. Phosphorylation of the IGF receptor as well as that of AKT was high in LTs. In addition, the expression of phosphomammalian target of rapamycin and phospho-glycogen synthase kinase $3{\beta}$ was increased and the expression of Forkhead box O3 was decreased in LTs. Therefore, we suggested that the IGF-1/AKT signaling pathway affects the formation, growth, and movement of the adductor muscle in triploid oysters.
Keywords
Triploid oyster; Adductor muscle; Muscle growth; Protein synthesis; Protein degradation;
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