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http://dx.doi.org/10.1186/s41240-019-0137-0

Characterization of antioxidative peptide purified from black eelpout (Lycodes diapterus) hydrolysate  

Lee, Jung Kwon (Department of Marine Biotechnology, Gangneung-Wonju National University)
Byun, Hee-Guk (Department of Marine Biotechnology, Gangneung-Wonju National University)
Publication Information
Fisheries and Aquatic Sciences / v.22, no.10, 2019 , pp. 22.1-22.7 More about this Journal
Abstract
The functional peptides from protein hydrolysates of various fishery sources have been identified such as antioxidant activity. The main intention of this study was purification and characterization of antioxidative peptide from black eelpout muscle. The antioxidative peptides were purified from black eelpout (Lycodes diapterus) muscle using different proteases. Antioxidant activity of black eelpout hydrolysates was evaluated using DPPH radical scavenging activity. Among six hydrolysates, the pepsin hydrolysate had the highest antioxidant activity compared to the other hydrolysates. Therefore, it was further purified and a peptide with seven amino acid residues of DLVKVEA (784 Da) was identified by amino acid sequence analysis. The EC50 value for scavenging DPPH radicals by purified peptide was 688.77 μM. Additionally, the purified peptide exhibited protective effect against DNA damage induces by oxidation in mouse macrophages (RAW 264.7 cells). The results of this study suggest that black eelpout muscle protein hydrolysate could potentially contribute to development of bioactive peptides in basic research.
Keywords
Antioxidant; DPPH radical scavenging; Peptide; Hydrolysates; Pepsin; Black eelpout muscle;
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