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http://dx.doi.org/10.1186/s41240-017-0052-1

Effects of astaxanthin on antioxidant capacity of golden pompano (Trachinotus ovatus) in vivo and in vitro  

Xie, Jia-jun (South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences)
Chen, Xu (South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences)
Niu, Jin (Institute of Aquatic Economic Animals, School of Life Science, Sun Yat-sen University)
Wang, Jun (South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences)
Wang, Yun (South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences)
Liu, Qiang-qiang (South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences)
Publication Information
Fisheries and Aquatic Sciences / v.20, no.4, 2017 , pp. 6.1-6.8 More about this Journal
Abstract
The objective of this research was to study the effect of astaxanthin (AST) on growth performance and antioxidant capacity in golden pompano (Trachinotus ovatus) both in vivo and in vitro. In the in vivo study, two diets were formulated with or without astaxanthin supplementation (D1 and D2; 0 and 200 mg/kg) to feed fish for 6 weeks. In the in vitro study, cells from hepatopancreas of golden pompano were isolated and four treatments with or without astaxanthin and $H_2O_2$ supplementation were applied (control group: without both astaxanthin and $H_2O_2$ treated; $H_2O_2$ group: just with $H_2O_2$ treated; $H_2O_2$ + AST group: with both astaxanthin and $H_2O_2$treated; AST group: just with AST treated). Results of the in vivo study showed that weight gain (WG) and special growth rate (SGR) significantly increased with astaxanthin supplemented (P < 0.05). Feed conversion ratio (FCR) of fish fed D2 diet was significantly lower than that of fish fed D1 diet (P < 0.05). Hepatic total antioxidant capacity (T-AOC) and the reduced glutathione (GSH) of golden pompano fed D2 diet were significant higher than those of fish fed D1 diet (P < 0.05). Superoxide dismutase (SOD) was significantly declined as astaxanthin was supplemented (P < 0.05). Results of the in vitro study showed that the cell viability of $H_2O_2$ group was 52.37% compared to the control group, and it was significantly elevated to 84.18% by astaxanthin supplementation ($H_2O_2$ + AST group) (P < 0.05). The total antioxidant capacity (T-AOC) and the reduced glutathione (GSH) of cell were significant decreased by oxidative stress from $H_2O_2$ (P < 0.05), but it could be raised by astaxanthin supplementation ($H_2O_2$ vs $H_2O_2$ + AST), and the malondialdehyde (MDA) was significant higher in $H_2O_2$ group (P < 0.05) and astaxanthin supplementation could alleviate the cells from lipid peroxidation injury. In conclusion, dietary astaxanthin supplementation can improve the growth performance of golden pompano. Moreover, astaxanthin can improve the golden pompano hepatic antioxidant capacity both in vivo and in vitro study by eliminating the reactive oxygen species.
Keywords
Golden pompano; Astaxanthin; Growth performance; Antioxidant capacity;
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