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http://dx.doi.org/10.1186/s41240-016-0008-x

Shrimp (Pandalopsis dispar) waste hydrolysate as a source of novel β-secretase inhibitors  

Li-Chan, Eunice C.Y. (Food, Nutrition & Health Program, Faculty of Land & Food Systems, The University of British Columbia)
Cheung, Imelda W.Y. (Food, Nutrition & Health Program, Faculty of Land & Food Systems, The University of British Columbia)
Byun, Hee-Guk (Department of Marine Biotechnology, Gangneung-Wonju National University)
Publication Information
Fisheries and Aquatic Sciences / v.19, no.2, 2016 , pp. 11.1-11.7 More about this Journal
Abstract
In this study, purified peptides from shrimp waste hydrolysates (SWHs) were examined for their inhibitory effects against ${\beta}$-secretase. During consecutive purification using a Sephadex G-25 column chromatography and high performance liquid chromatography on a C18 column, a potent ${\beta}$-secretase inhibitory peptide Asp-Val-Leu-Phe-His (629 Da) was isolated and identified from SWH24 by Q-TOF MS/MS and the $IC_{50}$ value was determined to be $92.70{\mu}M$. The ${\beta}$-secretase inhibition patterns of the purified peptides were found to be competitive. Among synthesized ${\beta}$-secretase inhibitory peptides, Leu-Phe-His had higher ${\beta}$-secretase inhibitory activity than the others. The result of this study suggests that the ${\beta}$-secretase inhibitory peptide derived from SWH24 could be potential candidates to develop nutraceuticals and pharmaceuticals.
Keywords
Alzheimer's disease; ${\beta}$-secretase inhibitory activity; Shrimp waste hydrolysates; Peptide;
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Times Cited By KSCI : 1  (Citation Analysis)
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