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http://dx.doi.org/10.5657/FAS.2015.0211

The Effects of Different Wavelengths of Light-Emitting Diodes on the Expression of Reproduction-Related Genes in Goldfish Carassius auratus  

Yun, Sung Gyu (Department of Science Education, Daegu University)
Kim, Na Na (Division of Marine BioScience, Korea Maritime and Ocean University)
Shin, Hyun Suk (Division of Marine BioScience, Korea Maritime and Ocean University)
Choi, Young Jae (Division of Marine BioScience, Korea Maritime and Ocean University)
Choi, Ji Yong (Division of Marine BioScience, Korea Maritime and Ocean University)
Song, Jin Ah (Division of Marine BioScience, Korea Maritime and Ocean University)
Choi, Cheol Young (Division of Marine BioScience, Korea Maritime and Ocean University)
Publication Information
Fisheries and Aquatic Sciences / v.18, no.2, 2015 , pp. 211-220 More about this Journal
Abstract
We investigated the differences in the expression of the neurohormones kisspeptin (Kiss) and gonadotropin-inhibitory hormone (GnIH) and cytochrome P450 aromatase (P450arom), gonadotropin hormones (GTHs), and sex steroids in the goldfish Carassius auratus exposed to light-emitting diodes (LEDs). The expression levels of Kiss1, Kiss2, G-protein-coupled receptor 54 (GPR54), GTHs, GnIH, and P450arom were compared between the control (white light) and LED-treated goldfish. Furthermore, we measured the plasma levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The levels of Kiss1 mRNA and protein; Kiss2, GPR54, and $GTH{\alpha}$ protein; GTH mRNA; and plasma FSH and LH in the hypothalamus and cultured hypothalamus cells were significantly higher in the green and purple LED treatment groups than in the other groups. These results suggested that red LEDs inhibit the sex maturation hormones, Kiss, GPR54, GTHs, and P450arom, and that GnIH plays a role in the negative regulation of reproductive function in goldfish.
Keywords
Cytochrome P450 aromatase; Gonadotropin hormones; Gonadotropin-inhibitory hormone; G protein-coupled receptor 54; Kisspeptin; Light-emitting diodes;
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