Browse > Article
http://dx.doi.org/10.5657/FAS.2013.0303

Development of Polymorphic Microsatellite Markers Suitable for Genetic Linkage Mapping of Olive Flounder Paralichthys olivaceus  

Kim, Woo-Jin (Biotechnology Research Division, National Fisheries Research and Development Institute)
Shin, Eun-Ha (Biotechnology Research Division, National Fisheries Research and Development Institute)
Kong, Hee Jeong (Biotechnology Research Division, National Fisheries Research and Development Institute)
Nam, Bo-Hye (Biotechnology Research Division, National Fisheries Research and Development Institute)
Kim, Young-Ok (Biotechnology Research Division, National Fisheries Research and Development Institute)
Jung, Hyungtaek (Biotechnology Research Division, National Fisheries Research and Development Institute)
An, Cheul Min (Biotechnology Research Division, National Fisheries Research and Development Institute)
Publication Information
Fisheries and Aquatic Sciences / v.16, no.4, 2013 , pp. 303-309 More about this Journal
Abstract
Microsatellite markers are important for gene mapping and for marker-assisted selection. Sixty-five polymorphic microsatellite markers were developed with an enriched partial genomic library from olive flounder Paralichthys olivaceus an important commercial fish species in Korea. The variability of these markers was tested in 30 individuals collected from the East Sea (Korea). The number of alleles for each locus ranged from 2 to 33 (mean, 17.1). Observed and expected heterozygosity as well as polymorphism information content varied from 0.313 to 1.000 (mean, 0.788), from 0.323 to 0.977 (mean, 0.820), and from 0.277 to 0.960 (mean, 0.787), respectively. Nine loci showed significant deviation from the Hardy-Weinberg equilibrium after sequential Bonferroni correction. Analysis with MICROCHECKER suggested the presence of null alleles at five of these loci with estimated null allele frequencies of 0.126-0.285. These new microsatellite markers from genomic libraries will be useful for constructing a P. olivaceus linkage map.
Keywords
Paralichthys olivaceus; Olive flounder; Microsatellite markers; Linkage map;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 An HS, Lee JH, Noh JK, Kim HC, Park CJ, Min BH and Myeong JI. 2010. Ten new microsatellite markers in cutlassfish Trichiurus lepturus derived from an enriched genomic library. Anim Cells Syst 14, 169-174.   과학기술학회마을   DOI   ScienceOn
2 Arora R, Lakhchaura BD, Prasad RB, Tantia MS and Vijh RK. 2004. Genetic diversity analysis of two buffalo populations of northern India using microsatellite markers. J Anim Breed Genet 121, 111-118. http://dx.doi.org/10.1111/j.1439-0388.2004.00451.x.   DOI   ScienceOn
3 Asahida T, Kobayashi T, Saitoh K and Nakayama I. 1996. Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea. Fish Sci 62, 727-730.   DOI   ScienceOn
4 Botstein D, White RL, Skolnick M and Davis RW. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32, 314-331.
5 Carleton KL, Streelman JT, Lee BY, Garnhart N, Kidd M and Kocher TD. 2002. Rapid isolation of CA microsatellites from the tilapia genome. Anim Genet 33, 140-144. http://dx.doi.org/10.1046/ j.1365-2052.2002.00817.x.   DOI   ScienceOn
6 Cho YG, Eun MY, McCouch SR and Chae YA. 1994. The semidwarf gene, sd-1, of rice (Oryza sativa L.). II. Molecular mapping and marker-assisted selection. Theor Appl Genet 89, 54-59.
7 Goldstein DB and Pollock DD. 1997. Launching microsatellites: a review of mutation processes and methods of phylogenetic interference. J Hered 88, 335-342.   DOI   ScienceOn
8 Hamilton MB, Pincus EL, Di Fiore A and Fleischer RC. 1999. A universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. Biotechniques 27, 500-502, 504-507.
9 Kim WJ, Kang JH, Nam BH, Kong HJ, Park EM, Lee JH, Kim KK, Kim YO, Han HS, Choi TJ and Cheong J. 2009. Development of 52 new polymorphic microsatellite markers for the olive flounder, Paralichthys olivaceus. Mol Ecol Resour 9, 839-842. http://dx.doi.org/10.1111/j.1755-0998.2009.02287.x.   DOI   ScienceOn
10 Kim WJ, Kim KK, Lee JH, Park DW, Park JY and Lee JY. 2003. Isolation and characterization of polymorphic microsatellite loci in the olive flounder (Paralichthys olivaceus). Mol Ecol Notes 3, 491-493. http://dx.doi.org/10.1046/j.1471-8286.2003.00524.x.   DOI   ScienceOn
11 Rice WR. 1989. Analyzing tables of statistical tests. Evolution 43, 223-225.   DOI   ScienceOn
12 Liu ZJ and Cordes JF. 2004. DNA marker technologies and their applications in aquaculture genetics. Aquaculture 238, 1-37. http://dx.doi.org/10.1016/j.aquaculture.2004.05.027.   DOI   ScienceOn
13 Marshall TC, Slate J, Kruuk LEB and Pemberton JM. 1998. Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7, 639-655. http://dx.doi.org/10.1046/j.1365-294x.1998.00374.x.   DOI   ScienceOn
14 O'Connel M and Wright JM. 1997. Microsatellite DNA in fishes. Rev Fish Biol Fish 7, 331-363. http://dx.doi.org/10.1023/A:1018443912945.   DOI
15 Takezaki N and Nei M. 1996. Genetic distances and reconstruction of phylogenetic trees from microsatellite DNA. Genetics 144, 389-399.
16 Van Oosterhout C, Hutchinson WF, Wills DPM and Shipley P. 2004. MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4, 535-538. http://dx.doi.org/10.1111/j.1471-8286.2004.00684.x   DOI   ScienceOn
17 Weir BS and Cockerham CC. 1984. Estimating F-statistics for the analysis of population structure. Evolution 38, 1358-1370.   DOI   ScienceOn
18 Zane L, Bargelloni L and Patarnello T. 2002. Strategies for microsatellite isolation: a review. Mol Ecol 11, 1-16. http://dx.doi.org/10.1046/j.0962-1083.2001.01418.x.   DOI   ScienceOn
19 Raymond M and Rousset F. 1995. GENEPOP (Version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86, 248-249.