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http://dx.doi.org/10.5657/FAS.2013.0093

The First Report of a Megalocytivirus Infection in Farmed Starry Flounder, Platichthys stellatus, in Korea  

Won, Kyoung-Mi (Southwest Sea Fisheries Research Institute, National Fisheries Research and Development Institute (NFRDI))
Cho, Mi Young (Aquatic Animal Disease Control Division, NFRDI)
Park, Myoung Ae (Aquatic Animal Disease Control Division, NFRDI)
Jee, Bo Young (Aquatic Animal Disease Control Division, NFRDI)
Myeong, Jeong-In (Aquaculture Management Division, NFRDI)
Kim, Jin Woo (Pathology Division, NFRDI)
Publication Information
Fisheries and Aquatic Sciences / v.16, no.2, 2013 , pp. 93-99 More about this Journal
Abstract
In 2009, a systemic megalocytivirus infection associated with high mortality was detected for the first time in cultured starry flounder Platichthys stellatus in Korea. Diseased starry flounder had pale bodies and gill coloring and enlarged spleens. Histopathological examinations revealed basophilic enlarged cells in various organs of diseased starry flounder. Polymerase chain reaction (PCR) was performed on tissue samples using three published primer sets developed for the red sea bream iridovirus. PCR products were detected for all primer sets, except 1-F/1-R, which are registered by the World Organization for Animal Health (OIE). The part of the gene corresponding to the full open reading frame encoding the viral major capsid protein (MCP) was amplified by PCR. PCR products of approximately 1,581 bp were cloned, and the nucleotide sequences were analyzed phylogenetically. The MCP gene of the starry flounder iridovirus, designated SFIV0909, was identical to that of the turbot reddish body iridovirus (AB166788).
Keywords
Starry flounder Platichthys stellatus; Red sea bream Iridovirus; Turbot reddish body iridovirus; Major capsid protein gene; Phylogenetic analysis;
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