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http://dx.doi.org/10.5657/FAS.2012.0325

Genomic Organization, Intronic Duplications, and Promoter Characteristics of the Fast Skeletal Myosin Light Chain-2 Gene (mlc2f) from Javanese Ricefish Oryzias javanicus  

Lee, Sang Yoon (Department of Marine Bio-Materials and Aquaculture, Pukyong National University)
Kim, Dong Soo (Department of Marine Bio-Materials and Aquaculture, Pukyong National University)
Nam, Yoon Kwon (Department of Marine Bio-Materials and Aquaculture, Pukyong National University)
Publication Information
Fisheries and Aquatic Sciences / v.15, no.4, 2012 , pp. 325-335 More about this Journal
Abstract
The present study characterized the fast skeletal myosin light chain-2 gene (mlc2f) in the euryhaline Javanese ricefish (Oryzias javanicus: Beloniformes). Coding nucleotide and deduced amino acid sequences of Javanese ricefish mlc2f were well conserved in the vertebrate lineage. Javanese ricefish mlc2f showed a typical seven-exon structure, and its promoter exhibited transcription factor binding motifs common to most muscle-specific genes. However, Javanese ricefish mlc2f also displayed tandem duplications of intronic sequences in both intron 1 and intron 3. Based on quantitative reverse transcription-polymerase chain reaction, the mlc2f transcripts were highly predominant in skeletal muscles of adults and were differentially modulated during embryonic development. Microinjection of the mlc2f promoter-driven red fluorescent protein (RFP) reporter construct successfully exhibited heterologous expression of the fluorescent reporter, primarily in muscular areas of hatchlings, although the distribution pattern of RFP signals was not uniform due to the mosaic nature of the introduced transgene. Data from this study indicate that the Javanese ricefish mlc2f gene has undergone "intra-intronic" duplication events in a species-specific manner and that the mlc2f regulator may also be useful in heterologous expression assays of the skeletal muscles of this species.
Keywords
Javanese ricefish; Oryzias javanicus; mlc2f gene and promoter; Duplication of intronic sequences; Microinjection;
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1 Ozernyuk ND, Nareiko VG, Smirnova YA and Zinov'eva RD. 2004. Pattern of skeletal muscle differentiation in fish: molecular biological approaches. Biol Bull 31, 209-215.   DOI
2 Sachdev S, Raychowdhury MK and Sarkar S. 2003. Human fast skeletal myosin light chain 2 cDNA: isolation, tissue specific expression of the single copy gene, comparative sequence analysis of isoforms and evolutionary relationships. DNA Seq 14, 339-350.   DOI
3 Song IY, Nam YK, Bang IC and Kim DS. 2010. Hybridization between marine medaka Oryzias dancena and Javanese medaka Oryzias javanicus. Korean J Fish Aquat Sci 43, 462-473.   과학기술학회마을   DOI   ScienceOn
4 Sweeney HL. 1995. Function of the N terminus of the myosin essential light chain of vertebrate striated muscle. Biophys J 68, 112S-118S.
5 Tang DD and Gunst SJ. 2004. The small GTPase Cdc42 regulates actin polymerization and tension development during contractile stimulation of smooth muscle. J Biol Chem 279, 51722-51728.   DOI   ScienceOn
6 Temple GK, Cole NJ and Johnston IA. 2001. Embryonic temperature and the relative timing of muscle-specific genes during development in herring (Clupea harengus L.). J Exp Biol 204, 3629-3637.
7 Wan H, He J, Ju B, Yan T, Lam TJ and Gong Z. 2002. Generation of two-color transgenic zebrafish using the green and red fluorescent protein reporter genes gfp and rfp. Mar Biotechnol 4, 146-154.   DOI
8 Watabe S. 1999. Myogenic regulatory factors and muscle differentiation during ontogeny in fish. J Fish Biol 55, 1-18.
9 Xu Y, He J, Tian HL, Chan CH, Liao J, Yan T, Lam TJ and Gong Z. 1999. Fast skeletal muscle-specific expression of a zebrafish myosin light chain 2 gene and characterization of its promoter by direct injection into skeletal muscle. DNA Cell Biol 18, 85-95.   DOI   ScienceOn
10 Xu Y, He J, Wang X, Lim TM and Gong Z. 2000. Asynchronous activation of 10 muscle-specific protein (MSP) genes during zebrafish somitogenesis. Dev Dyn 219, 201-215.   DOI   ScienceOn
11 Black BL and Olson EN. 1998. Transcriptional control of muscle development by myocyte enhancer factor-2 (MEF2) proteins. Annu Rev Cell Dev Biol 14, 167-196.   DOI   ScienceOn
12 Carvalho L and Heisenberg CP. 2010. The yolk syncytial layer in early zebrafish development. Trends Cell Biol 20, 586-592.   DOI   ScienceOn
13 Cho YS, Lee SY, Kim YK, Kim DS and Nam YK. 2011. Functional ability of cytoskeletal $\beta$-actin regulator to drive constitutive and ubiquitous expression of a fluorescent reporter throughout the life cycle of transgenic marine medaka Oryzias dancena. Transgenic Res 20, 1333-1355.   DOI   ScienceOn
14 Chu WY, Chen J, Zhou RX, Zhao FL, Meng T, Chen DX, Nong XX, Liu Z, Lu SQ and Zhang JS. 2011. Characterization and ontogenetic expression analysis of the myosin light chains from the fast white muscle of mandarin fish Siniperca chuatsi. J Fish Biol 78, 1225-1238.   DOI   ScienceOn
15 Czosnek H, Nudel U, Shani M, Barker PE, Pravtcheva DD, Ruddle FH and Yaffe D. 1982. The genes coding for the muscle contractile proteins, myosin heavy chain, myosin light chain 2, and skeletal muscle actin are located on three different mouse chromosomes. EMBO J 1, 1299-1305.
16 Du SJ, Gao J and Anyangwe V. 2003. Muscle-specific expression of myogenin in zebrafish embryos is controlled by multiple regulatory elements in the promoter. Comp Biochem Physiol B Biochem Mol Biol 134, 123-134.   DOI   ScienceOn
17 Esser K, Nelson T, Lupa-Kimball V and Blough E. 1999. The CACC box and myocyte enhancer factor-2 sites within the myosin light chain 2 slow promoter cooperate in regulating nerve-specific transcription in skeletal muscle. J Biol Chem 274, 12095-12102.   DOI   ScienceOn
18 Gabillard JC, Ralliere C, Sabin N and Rescan PY. 2010. The production of fluorescent transgenic trout to study in vitro myogenic cell differentiation. BMC Biotechnol 10, 39.   DOI   ScienceOn
19 Fujita K, Ye LH, Sato M, Okagaki T, Nagamachi Y and Kohama K. 1999. Myosin light chain kinase from skeletal muscle regulates an ATP-dependent interaction between actin and myosin by binding to actin. Mol Cell Biochem 190, 85-90.   DOI   ScienceOn
20 Funkenstein B, Skopal T, Rapoport B, Rebhan Y, Du SJ and Radaelli G. 2007. Characterization and functional analysis of the 5′ flanking region of myosin light chain-2 gene expressed in white muscle of the gilthead sea bream (Sparus aurata). Comp Biochem Physiol Part D Genomics Proteonomics 2, 187-199.   DOI   ScienceOn
21 Galloway TF, Bardal T, Kvam SN, Dahle SW, Nesse G, Randol M, Kjorsvik E and Andersen O. 2006. Somite formation and expression of MyoD, myogenin and myosin in Atlantic halibut (Hippoglossus hippoglossus L.) embryos incubated at different temperatures: transient asymmetric expression of MyoD. J Exp Biol 209, 2432-2441.   DOI   ScienceOn
22 Hall TE, Cole NJ and Johnston IA. 2003. Temperature and the expression of seven muscle-specific protein genes during embryogenesis in the Atlantic cod Gadus morhua L. J Exp Biol 206, 3187-3200.   DOI   ScienceOn
23 Ikebe M, Kambara T, Stafford WF, Sata M, Katayama E and Ikebe R. 1998. A hinge at the central helix of the regulatory light chain of myosin is critical for phosphorylation-dependent regulation of smooth muscle myosin motor activity. J Biol Chem 273, 17702- 17707.   DOI   ScienceOn
24 Ju B, Chong SW, He J, Wang X, Xu Y, Wan H, Tong Y, Yan T, Korzh V and Gong Z. 2003. Recapitulation of fast skeletal muscle development in zebrafish by transgenic expression of GFP under the mylz2 promoter. Dev Dyn 227, 14-26.   DOI   ScienceOn
25 Kubista M, Andrade JM, Bengtsson M, Forootan A, Jonak J, Lind K, Sindelka R, Sjoback R, Sjogreen B, Strombom B, Stahlberg A and Zoric N. 2006. The real-time polymerase chain reaction. Mol Aspects Med 27, 95-125.   DOI   ScienceOn
26 Kobiyama A, Nihei Y, Hirayama Y, Kikuchi K, Suetake H, Johnston IA and Watabe S. 1998. Molecular cloning and developmental expression patterns of the Myod and MEF2 families of muscle transcription factors in the carp. J Exp Biol 201, 2801-2813.
27 Koyama J, Kawamata M, Imai S, Fukunaga M, Uno S and Kakuno A. 2008. Java medaka: a proposed new marine test fish for ecotoxicology. Environ Toxicol 23, 487-491.   DOI   ScienceOn
28 Krasnov A, Teerijoki H, Gorodilov Y and Molsa H. 2003. Cloning of rainbow trout (Oncorhynchus mykiss) $\alpha$-actin, myosin regulatory light chain genes and the 5′-flanking region of $\alpha$-tropomyosin. Functional assessment of promoters. J Exp Biol 206, 601-608.   DOI   ScienceOn
29 Lehtinen SK, Rahkila P, Helenius M, Korhonen P and Salminen A. 1996. Down-regulation of transcription factors AP-1, Sp-1, and $NF-{\kappa}B$ precedes myocyte differentiation. Biochem Biophys Res Commun 229, 36-43.   DOI   ScienceOn
30 Moutou KA, Canario AVM, Mamuris Z and Power DM. 2001. Molecular cloning and sequence of Sparus aurata skeletal myosin light chains expressed in white muscle: developmental expression and thyroid regulation. J Exp Biol 204, 3009-3018.
31 Mugue NS and Ozernyuk ND. 2006. Comparative structural analysis of myosin light chains and gene duplication in fish. Biol Bull 33, 30-34.   DOI
32 Mugue NS, Tikhonov AV and Ozernyuk ND. 2005. Ontogenetic and phylogenetic analysis of myosin light chain proteins from skeletal muscles of loach Misgurnus fossilis. Biol Bull 32, 473-477.   DOI