Browse > Article
http://dx.doi.org/10.5657/fas.2009.12.2.098

Functional Evaluation of the Rockbream (Oplegnathus fasciatus) Beta-actin Promoter as a Candidate Regulatory Element for DNA Vaccination  

Kosuke, Zenke (Department of Aquatic Life Medicine, Pukyong National University)
Lee, Sang-Yoon (Department of Aquaculture, Pukyong National University)
Kim, Ki-Hong (Department of Aquatic Life Medicine, Pukyong National University)
Nam, Yoon-Kwon (Department of Aquaculture, Pukyong National University)
Publication Information
Fisheries and Aquatic Sciences / v.12, no.2, 2009 , pp. 98-103 More about this Journal
Abstract
The potential utility of the rockbream (Oplegnathus fasciatus) $\beta$-actin 5'-upstream sequence as a regulatory element for DNA vaccination was evaluated based on in vitro and in vivo heterologous expression assays. In the in vitro transfection experiment, the efficacy of the rockbream $\beta$-actin promoter to drive the expression of a downstream lacZ gene was significantly higher (more than fourfold) than that of the human cytomegalovirus (hCMV) promoter in two fish cell lines (grunt Haemulon plumierii fin and bluegill Lepomis macrochirus fry cell lines). In contrast, the functional activity of the rockbream $\beta$-actin promoter was hardly detectable in a mammalian mouse embryonic fibroblast cell line. Rockbream skeletal muscles injected in vivo with a GFP reporter construct driven by the $\beta$-actin promoter displayed the significantly higher expression of a GFP protein (more than threefold) than did those injected with hCMV promoter driven construct. Data from this study suggest that the homologous rockbream $\beta$-actin promoter could be used as a potential regulator for DNA vaccination in this species.
Keywords
Rockbream (Oplegnathus fasciatus); $\beta$-Actin regulator; DNA vaccine; Heterologous expression;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Hwang, G.L., A. Rahman, A. Razak, F. Shom, H. Frahmand, A. Smith, C. Brooks and N. Maclean. 2003. Isolation and characterization of tilapia $\beta$-actin promoter and comparison of its activity with carp $\beta$-actin promoter. Biochim. Biophys. Acta, 1125, 11-18
2 Mikalsen, A.B., H. Sindre, J. Torgersen and E. Rimstad. 2005. Protective effects of a DNA vaccine expressing the infectious salmon anemia vims hemagglutininesterase in Atlantic salmon. Vaccine, 23, 4895-4905   DOI   ScienceOn
3 Nam, Y.K., N. Maclean, G. Hwang and D.S. Kim. 2008. Autotransgenic and allotransgenic manipulation of growth traits in fish for aquaculture. J. Fish Biol., 72, 1-26   DOI
4 Nam, Y.K., J.K. Noh, Y.S. Cho, H.J. Cho, K.N. Cho, C.G. Kim and D.S. Kim. 2001. Dramatically accelerated growth and extraordinary gigantism of transgenic mud loach Misgurnus mizolepis. Transgenic Res., 10, 353-362   DOI   ScienceOn
5 Noh, J.K., K.N. Cho, E.H. Han, A. Kim, J.S. Lee, D.S. Kim and C.G. Kim. 2003. Genomic cloning of mud loach Misgíμrnus mizolepis (Cypriniformes, Cobitidae) $\beta$-actin gene and usefulness of its promoter region for fish transgenesis. Mar. Biotechnol., 5, 244- 252   DOI   ScienceOn
6 Choi, S.K., S.R. Kwon, Y.‘K. Nam, S.K. Kim and K.H Kim. 2006. Organ distribution of red sea bream iridovims (RSIV) DNA in asymptomatic yearling and fingerling rock bream (Oplegnathus fasciatus) and effects of water temperature on transition of RSIV into acute phase. Aquaculture, 256, 23-26   DOI   ScienceOn
7 Lee, S.Y., K.H. Kim and Y.K. Nam. 2009. Molecular characterization of rockbream (Oplegnathus fasciatus) cytoskeletal $\beta$-actin gene and 5' -upstream flaking region. J. Aquat. Fish. Sci. (in press)
8 Recce, K.S., D. McElroy and R. Wu. 1992. Function and evolution of actins. Evol. Biol., 26, 1-34
9 Gomez-Chiarii, M. and L.A. Chiaverini. 1999. Evaluation of eukaryotic promoters for the constmction of DNA vaccines for aquaculture. Genet. Anal. Biomol. Eng., 15, 121-124   DOI   ScienceOn
10 Chan, W.K. and R.H. Devlin. 1993. Polymerase chain reaction amplification and functional characterization of sockeye salmon histone H3, metallothionein-B and protaminc promoters. Mol. Mar. Biol. Biotechnol., 2, 308-318   PUBMED
11 Winkler, C., Y. Hong, J. Wittbrodt and M. Schartl. 1992 Analysis of hcterologous and homologous promoters and cnhancers in vitro and in vivo by gεne transfer into Japanese medaka (Oryzias latpees) and Xiphophorus. Mol. Mar. Biol. Biotcchnol., 1, 326-327
12 Sanchez, E., J. Coll and C. Tafalla. 2007. Expression of ìnducible CC chemokines in rainbow trout (Oncorhynchus mykiss) in rcsponse to a viral heamorrhagic scpticemia vims (VHSV) DNA vaccine and interlcukin 8. Dev. Comp. Tmmunol., 31, 916-926   DOI   ScienceOn
13 Verri, T., L. Ingrosso, R. Chiloiro, A. Danieli, V. Zonno, P. Alifano, N. Romano, G. Scapigliati, S. Viella and C. Storelli. 2003. Asscssment of DNA vaccine potential for gilthead sea bream (Sparus aurata) by intramuscular injection of reporter gene. Fish Shellfish lmmunol., 15, 283-295   DOI   ScienceOn
14 Pasnik, D.J. and S.A. Smith, 2005. lmmunogenic and protective effects of a DNA vaccinc for Mycobacterium marinum in fish. Vet. lmmunol. Immunophathol., 103, 195-206   DOI   ScienceOn
15 Adams, A. and K.D. Thompson. 2006. Biotechnology offers revolution to fish health management. Trends. Bio-tech., 24, 201-205   DOI   ScienceOn
16 Robertson, J.S. and E. Griffths. 2006. Assuring the quality, safety, and cfficacy of DNA vaccincs. Mcthods Mol Med., 127, 363-374
17 Garver, K.A., S.E. LaPatra and G. Kurath. 2005. Efficacy of an infectious hematopoietic necrosis (IHN) vims DNA vaccine in Chinook Oncorhynchus tshawytscha and sockeye O. nerka salmon. Dis. Aquat. Org., 64, 13-22   DOI
18 Lorenzen, N. and S.E. LaPatra. 2005. DNA vaccines for aquacultured fish. Rev. Sci. Tech., 24, 201-213   DOI   PUBMED
19 Ruiz, S., C. Tafalla, A. Cuesta， A. Estepa and J.M. Coll. 2008. ln vitro search for altemative promoters to the human immediate early cytomegalovims (IE-CMV) to exprcss thc G gcnc of viral hacmorrhagic septicemia vims (VHSV) in fish epithelial cells. Vaccine, 26, 6620-6629   DOI   ScienceOn
20 Hackett, P.B. and M.C. Alvarez. 2000. The molecular genetics of transgenic fish. In: Recent Advances in Marine Biotechnology, Fingerman, M. and R. Nagabhushanam. eds. Science Publishers Inc., New Hampshire, 77-145
21 Heppell, J. and H.L. Davis. 2000. Application of DNA vaccine technology to aquaculture. Adv. Dmg Del. Rev., 43, 29-43   DOI   ScienceOn