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http://dx.doi.org/10.5657/fas.2008.11.4.209

Detection of Heterotrophic Dinoflagellate Pfiesteria piscicida (Dinophyceae) in Surface Water Samples Using Real-time PCR  

Park, Tae-Gyu (Marine Ecology Research Division, National Fisheries Research and Development Institute)
Kang, Yang-Soon (Marine Ecology Research Division, National Fisheries Research and Development Institute)
Seo, Mi-Kyung (Marine Ecology Research Division, National Fisheries Research and Development Institute)
Park, Young-Tae (Marine Ecology Research Division, National Fisheries Research and Development Institute)
Publication Information
Fisheries and Aquatic Sciences / v.11, no.4, 2008 , pp. 209-211 More about this Journal
Abstract
Heterotrophic dinoflagellate Pfiesteria piscicida (Dinophyceae) has been claimed to produce potent ichthyotoxins that cause disorientation and eventually death of fish and other marine animals. A real-time PCR probe targeting for SSU rRNA gene was used for detection of P. piscicida in Chinhae Bay, Korea. PCR inhibitors were successfully removed by dilution of template DNA. Positive detections were shown from surface water samples indicating the presence of P. piscicida in Chinhae Bay.
Keywords
Harmful algal blooms; Pfiesteria piscicida; real-time PCR; red tide;
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